Blomlöf L, Otteskog P, Hammarström L
Scand J Dent Res. 1981 Apr;89(2):180-7. doi: 10.1111/j.1600-0722.1981.tb01669.x.
The viability of the periodontal ligament (PDL) cells is critical for the successful healing of replanted exarticulated teeth. It is mainly dependent on the duration of the extra-alveolar time and the storage medium. Saliva has usually been recommended as the most suitable storage medium, but recent experimental studies indicate that milk is preferable. In the present study the effect on cultured PDL cells of saliva and milk has been compared with some reference media such as tap water or saline by means of a 3H-uridine leakage test. Storage in milk or saline was found to cause much less 3H-uridine leakage than storage in saliva or tap water. Cells stored in milk for 60-180 min showed about the same leakage as cells stored in saline or Hanks' balanced salt solution. Osmolality measurements showed that saliva was hypotonic, while the osmolality of milk ranged within physiological limits. When the osmolality of saliva was increased by addition of NaCl the leakage of the stored cells decreased to the level of cells stored in 0.9% NaCl or milk.
牙周膜（PDL）细胞的活力对于再植离体牙的成功愈合至关重要。它主要取决于牙槽外时间的长短和储存介质。唾液通常被推荐为最合适的储存介质，但最近的实验研究表明牛奶更优。在本研究中，通过3H-尿苷泄漏试验，将唾液和牛奶对培养的PDL细胞的影响与一些参考介质（如自来水或生理盐水）进行了比较。发现储存在牛奶或生理盐水中比储存在唾液或自来水中引起的3H-尿苷泄漏要少得多。储存在牛奶中60 - 180分钟的细胞显示出与储存在生理盐水或汉克斯平衡盐溶液中的细胞大致相同的泄漏情况。渗透压测量表明，唾液是低渗的，而牛奶的渗透压在生理范围内。当通过添加氯化钠提高唾液的渗透压时，储存细胞的泄漏减少到储存在0.9%氯化钠或牛奶中的细胞水平。
