Lindskog S, Blomlöf L, Hammarström L
Scand J Dent Res. 1983 Dec;91(6):465-72. doi: 10.1111/j.1600-0722.1983.tb00847.x.
Milk and saliva were tested in vitro as potential storage media for avulsed teeth. Developing monkey teeth were extracted and stored in milk or saliva for periods ranging from 1 to 6 h. The osmolality, pH, conductivity and number of viable bacteria in the media were determined after predetermined intervals during the storage periods. After the storage periods the teeth were either prepared for scanning electron microscopy or cultured for 24 h in Eagle's medium supplemented with 3H-thymidine. In the scanning electron microscope numerous adherent bacteria were seen covering the periodontal membrane after storage in saliva but none were found after storage in milk. The cultured teeth were sectioned and evaluated with autoradiography. Superficial parts of the periodontal membrane were rapidly injured by storage in saliva while the epithelial root sheath and the apical pulpal cells were affected at a later stage. Cells neighboring the cementoblasts incorporated 3H-thymidine after 6 h storage in milk but not after storage in saliva for the same length of time. It was concluded that the low osmolality in combination with bacteria which adhered to the periodontal membrane made saliva less suited than milk for long time storage of avulsed teeth. Furthermore, a viable layer of cells close to the root surface seemed to be a prerequisite for a successful healing without root resorption after replantation.
对牛奶和唾液作为脱位牙潜在储存介质进行了体外测试。拔除正在发育的猴牙，将其储存在牛奶或唾液中1至6小时。在储存期间的预定间隔后，测定介质中的渗透压、pH值、电导率和活菌数量。储存期结束后，将牙齿要么制备用于扫描电子显微镜检查，要么在补充有3H-胸腺嘧啶核苷的伊格尔培养基中培养24小时。在扫描电子显微镜下，唾液储存后可见大量附着细菌覆盖牙周膜，而牛奶储存后未发现细菌。对培养的牙齿进行切片并用放射自显影术评估。牙周膜的表层在唾液储存时迅速受损，而上皮根鞘和根尖牙髓细胞在后期受到影响。在牛奶中储存6小时后，成牙骨质细胞附近的细胞摄取了3H-胸腺嘧啶核苷，但在相同时间的唾液储存后未摄取。得出的结论是，低渗透压与附着在牙周膜上的细菌相结合，使得唾液比牛奶更不适合长时间储存脱位牙。此外，靠近牙根表面的一层活细胞似乎是再植后成功愈合且无牙根吸收的先决条件。
