Kamada N, Dohy H, Okada K, Oguma N, Kuramoto A, Tanaka K, Uchino H
Blood. 1981 Dec;58(6):1213-7.
Cytogenetic studies were made on 160 patients with acute nonlymphocytic leukemia (ANLL) between 1963 and 1979, of whom 115 had acute myelocytic leukemia with 67 patients showing aneuploidy (58.3%). Among these, 24 patients were found to have similar chromosome alterations that appeared to involve specifically chromosomes 8 and 21. Banding studies on at least 7 of these patients confirmed the presence of a translocation between these two chromosomes. Of 160 ANLL patients, 142 were scored for neutrophil alkaline phosphatase (neutrophil AP) at the time of diagnosis. Fifty-nine patients showed a low neutrophil AP score, 42 a normal value, and 41 a high value. All patients with 8;21 (or C/G) translocation had a low neutrophil AP score and leukemic cells with maturation (M2 of FAB classification) in the bone marrow. In vitro liquid culture for 2 wk of 8;21 translocated leukemic cells revealed no increase of neutrophil AP activity nor increase of mature granulocytes, whereas 9;22 translocated chronic myelocytic leukemia cells with a low neutrophil AP score did so. Neutrophil AP score at the time of diagnosis in acute myelocytic leukemia is very useful for detecting 8;21 translocation AML and for studying the pathophysiology and genetic alterations of the characteristic subgroup of AML with 8'21 translocation.
1963年至1979年间，对160例急性非淋巴细胞白血病（ANLL）患者进行了细胞遗传学研究，其中115例为急性髓细胞白血病，67例（58.3%）显示非整倍体。其中，发现24例患者有相似的染色体改变，似乎特别涉及8号和21号染色体。对其中至少7例患者的显带研究证实了这两条染色体之间存在易位。在160例ANLL患者中，142例在诊断时进行了中性粒细胞碱性磷酸酶（中性粒细胞AP）评分。59例患者中性粒细胞AP评分低，42例正常，41例高。所有8；21（或C/G）易位的患者中性粒细胞AP评分低，骨髓中有成熟的白血病细胞（FAB分类的M2）。对8；21易位的白血病细胞进行2周的体外液体培养，未发现中性粒细胞AP活性增加，也未发现成熟粒细胞增加，而中性粒细胞AP评分低的9；22易位的慢性髓细胞白血病细胞则有此现象。急性髓细胞白血病诊断时的中性粒细胞AP评分对于检测8；21易位急性髓细胞白血病以及研究8'21易位急性髓细胞白血病特征亚组的病理生理学和基因改变非常有用。
