Owens G K, Rabinovitch P S, Schwartz S M
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7759-63. doi: 10.1073/pnas.78.12.7759.
Arteries of hypertensive animals have a greater mass of smooth muscle than those of normotensive controls. We examined the contribution of smooth muscle cell hypertrophy and hyperplasia to this increase in mass. Cell size measurements obtained by (i) image analysis of enzyme-dispersed cells, (ii) morphometric evaluation of tissue sections, and (iii) biochemical measures of protein/cell and actin/cell ratios on isolated cells showed that average cell size was greater in spontaneously hypertensive rats than in normotensive Wistar-Kyoto and Sprague-Dawley controls. Average DNA/cell ratios were also increased in spontaneously hypertensive rats while protein/DNA ratios were not different. Analysis of nuclear DNA content of individual cells by flow microfluorimetry and Feulgen-DNA microdensitometry measurements showed that greater than 20% of spontaneously hypertensive rats cells were polyploid while less than 10% of control cells were polyploid. Estimates of cell number per centimeter of aortic length, based on ploidy and DNA content, show no difference between control and hypertensive rats. Thus, smooth muscle hypertrophy alone accounts for the increased mass of smooth muscle in aortas of spontaneously hypertensive rats. Furthermore, this cellular hypertrophy is accompanied by a change in nuclear ploidy. This nuclear response in hypertension may represent a fixed change related to the establishment of a chronic hypertensive state.
高血压动物的动脉平滑肌质量比正常血压对照动物的更大。我们研究了平滑肌细胞肥大和增生对这种质量增加的作用。通过以下方法获得的细胞大小测量结果：(i) 对酶分散细胞进行图像分析，(ii) 对组织切片进行形态计量学评估，以及 (iii) 对分离细胞的蛋白质/细胞和肌动蛋白/细胞比率进行生化测量，结果显示自发性高血压大鼠的平均细胞大小大于正常血压的Wistar-Kyoto大鼠和Sprague-Dawley对照大鼠。自发性高血压大鼠的平均DNA/细胞比率也增加，而蛋白质/DNA比率没有差异。通过流式细胞荧光测定法和福尔根DNA显微密度测定法对单个细胞的核DNA含量进行分析表明，超过20%的自发性高血压大鼠细胞是多倍体，而对照细胞中只有不到10%是多倍体。基于倍性和DNA含量对每厘米主动脉长度的细胞数量进行估计，结果显示对照大鼠和高血压大鼠之间没有差异。因此，单纯平滑肌肥大就导致了自发性高血压大鼠主动脉中平滑肌质量的增加。此外，这种细胞肥大伴随着核倍性的变化。高血压中的这种核反应可能代表与慢性高血压状态建立相关的一种固定变化。
