Whinnery M A, Shaw J O, Beck N
Am J Physiol. 1982 Mar;242(3):F220-5. doi: 10.1152/ajprenal.1982.242.3.F220.
The production of prostaglandin E2- (PGE2) like and thromboxane A2-(TXA2) like substances is increased after release of unilateral ureteral obstruction (UUO) for 3 days in the isolated perfused rabbit kidney. It has been postulated that this increase in TXA2 biosynthesis might contribute to the development of vasoconstriction in the obstructed kidney. In the present studies, the production of TXA2 and PGE2 in the kidney was further investigated in rats after UUO for 2-18 h. Radioimmunoassay was used to determine thromboxane B2 (TXB2), a chemically stable metabolite of TXA2, and PGE2 production during the incubation of renal slices in vitro. Unlike previous studies, an increase in TXB2 and PGE2 production was demonstrable in the obstructed kidney even in the absence of pharmacological stimulation by bradykinin or angiotensin II. The effect of UUO on prostaglandin production differed in the different anatomical parts of the kidney. In the papilla, production of both TXB2 and PGE2 was increased in the obstructed kidney. In the cortex, however, UUO had a stimulatory effect only on TXB2 production but not on PGE2 production. The increase in TXB2 and PGE2 production was demonstrable as early a 2 h (tested) after ureteral obstruction. Prolongation of ureteral obstruction for 18 h diminished the stimulatory effect of UUO on PGE2 production but not on TXB2 production.
在离体灌注的兔肾中，单侧输尿管梗阻（UUO）解除3天后，前列腺素E2样物质和血栓素A2样物质的生成增加。据推测，血栓素A2生物合成的这种增加可能有助于梗阻肾血管收缩的发展。在本研究中，进一步研究了UUO 2 - 18小时后大鼠肾脏中血栓素A2和前列腺素E2的生成情况。采用放射免疫分析法测定血栓素B2（TXB2，血栓素A2的化学稳定代谢产物）以及肾切片在体外孵育期间前列腺素E2的生成。与先前的研究不同，即使在没有缓激肽或血管紧张素II的药理刺激的情况下，梗阻肾中TXB2和前列腺素E2的生成也明显增加。UUO对前列腺素生成的影响在肾脏的不同解剖部位有所不同。在肾乳头，梗阻肾中TXB2和前列腺素E2的生成均增加。然而，在皮质中，UUO仅对TXB2的生成有刺激作用，而对前列腺素E2的生成没有影响。输尿管梗阻后2小时（检测时间）即可明显看出TXB2和前列腺素E2生成增加。输尿管梗阻延长至18小时会减弱UUO对前列腺素E2生成的刺激作用，但对TXB2的生成没有影响。
