Schreibman M P, Berkowitz E J, van den Hurk R
Cell Tissue Res. 1982;224(1):781-7. doi: 10.1007/BF00217268.
The gonads of 3-day- to 7-month-old male and female platyfish (Xiphophorus maculatus) were examined for the presence of delta 5-3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and glucose-6-phosphate dehydrogenase (G6PD) by histochemical means. In 3-day-old males a positive response for both enzymes is localized in the Leydig cells. With subsequent testicular development, these cells increase in number and display greater activity at the periphery of the testis and around the efferent ducts. In 3-day-old females 3 beta-HSD and G6PD are localized in the stromal cells of the ovary. These cells increase in number and activity as the animals become sexually mature. Sertoli cells, efferent duct epithelium, and ovarian granulosa cells are negative at all stages of development examined. Our findings suggest that the gonads of neonatal fish possess the potential for steroidogenesis. The role played by sex-steroid hormones in the maturation of the brain-pituitary-gonad axis is discussed.
采用组织化学方法，对3日龄至7月龄的雄性和雌性剑尾鱼（Xiphophorus maculatus）性腺进行检测，以确定是否存在δ5-3β-羟基类固醇脱氢酶（3β-HSD）和葡萄糖-6-磷酸脱氢酶（G6PD）。在3日龄雄性个体中，两种酶的阳性反应均定位于睾丸间质细胞。随着睾丸的后续发育，这些细胞数量增加，并在睾丸周边和输出小管周围表现出更强的活性。在3日龄雌性个体中，3β-HSD和G6PD定位于卵巢的基质细胞。随着动物性成熟，这些细胞数量和活性增加。在所检查的发育各个阶段，支持细胞、输出小管上皮细胞和卵巢颗粒细胞均呈阴性。我们的研究结果表明，新生鱼的性腺具有类固醇生成的潜力。文中讨论了性类固醇激素在脑-垂体-性腺轴成熟过程中所起的作用。
