Hiscott J, Cantell K, Weissmann C
Nucleic Acids Res. 1984 May 11;12(9):3727-46. doi: 10.1093/nar/12.9.3727.
We developed a method for quantitating closely related mRNAs by S1 mapping and used it to determine the levels of mRNAs for IFN-beta, IFN-gamma and various alpha IFNs (IFN-alpha 1, -alpha 2, -alpha 4, -alpha 5, -alpha 6, -alpha 7, -alpha 8 and -alpha 14) in human peripheral blood leukocytes, lymphoblastoid (Namalwa), HeLa and human fibroblastic cells, induced in different fashions. The ratio of alpha to beta IFN transcripts varied greatly, depending on the cell type. The levels of the individual IFN-alpha RNAs were very different: IFN-alpha 1, -alpha 2 and -alpha 4 RNAs constituted the major fraction of the IFN-alpha transcripts measured. Moreover, there was a striking difference in the proportion of individual IFN-alpha mRNA species in different cell types. Use of different induction protocols did not significantly affect the proportion of IFN mRNAs. IFN production was not proportional to mRNA level in all cases, as lymphoblastoid cells induced by incubation at high density and virus-induced HeLa cells contained high levels of IFN-beta but produced little antiviral activity.
我们开发了一种通过S1核酸酶图谱分析来定量密切相关mRNA的方法，并利用该方法测定了以不同方式诱导的人外周血白细胞、淋巴母细胞样（Namalwa）细胞、HeLa细胞和人成纤维细胞中IFN-β、IFN-γ以及各种α干扰素（IFN-α1、-α2、-α4、-α5、-α6、-α7、-α8和-α14）的mRNA水平。α干扰素与β干扰素转录本的比例因细胞类型而异，差异很大。各个IFN-α RNA的水平差异也非常大：IFN-α1、-α2和-α4 RNA构成了所测IFN-α转录本的主要部分。此外，不同细胞类型中各个IFN-α mRNA种类的比例存在显著差异。使用不同的诱导方案对IFN mRNA的比例没有显著影响。在所有情况下，IFN的产生与mRNA水平并不成正比，因为高密度培养诱导的淋巴母细胞样细胞和病毒诱导的HeLa细胞含有高水平的IFN-β，但产生的抗病毒活性很少。
