一种用于胸腺嘧啶二聚体的灵敏放射免疫测定法。
A sensitive radioimmuno assay for thymine dimers.
作者信息
Klocker H, Auer B, Burtscher H J, Hofmann J, Hirsch-Kauffmann M, Schweiger M
出版信息
Mol Gen Genet. 1982;186(4):475-7. doi: 10.1007/BF00337950.
A sensitive radioimmuno assay (RIA) method for detection of the UV photoproduct, thymine dimers (TT) has been developed. The limit of detection of this method is 6 X 10(-14) mol or 15 pg thymine dimer. It is highly specific: A structurally similar compound such as uridine dimer (UU) interferes with the detection of thymine dimers only when it is 53,000-fold or more in molar excess. Since this RIA method does not require the use of labeled DNA, it represents a considerable improvement for repair studies with radiation-sensitive cells.
已开发出一种用于检测紫外线光产物胸腺嘧啶二聚体（TT）的灵敏放射免疫测定（RIA）方法。该方法的检测限为6×10⁻¹⁴摩尔或15皮克胸腺嘧啶二聚体。它具有高度特异性：结构相似的化合物如尿苷二聚体（UU）只有在摩尔过量53000倍或更多时才会干扰胸腺嘧啶二聚体的检测。由于这种RIA方法不需要使用标记的DNA，它对于对辐射敏感的细胞的修复研究而言是一项重大改进。
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