Wood Eilidh M, Hornaday Kylie K, Newton Matthew, Wang Melinda, Wood Stephen L, Slater Donna M
Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
Department of Obstetrics and Gynaecology, Cumming School of Medicine, University of Calgary, Calgary, Alberta, Canada.
PLoS One. 2025 Jul 14;20(7):e0315484. doi: 10.1371/journal.pone.0315484. eCollection 2025.
Prostaglandins and other related molecules in the eicosanoid family have long been implicated in the process of both term and preterm labour. Although, exactly which eicosanoids are involved and whether they have utility as biomarkers for labour, remains to be shown. The objective of this study was to determine whether urinary prostaglandins and related molecules a) change with labour and/or cervical changes, at term and preterm, and/or b) are associated with timing of delivery in individuals with threatened preterm labour. Pregnant individuals were recruited into the following groups: n = 32 term non-labour, n = 49 term labour, n = 15 preterm non-labour controls, n = 43 threatened preterm labour with preterm delivery, and n = 44 threatened preterm labour with term delivery. Metabolites of prostaglandins PGE2, PGF2α, PGD2, and PGI2 as well as 8-isoprostane were measured by ELISA. In addition, in a small (n = 24) subset of samples, 147 eicosanoids were measured using a mass-spectrometry based targeted lipidomics panel. At term labour prostaglandin PGF2α and PGE2 and PGF2α metabolites were increased compared to term non-labour. There were no changes in any prostaglandin metabolites prior to labour onset. Prostaglandin I2 metabolite was lower in individuals with threatened preterm labour who delivered preterm compared to those who went on to deliver at term. In our discovery cohort, we identified 20 additional eicosanoids as highly expressed in maternal urine, include members of the prostaglandin, hydroxyeicosatetraenoic acid (HETE), epoxyeicosatrienoic acid (EET), dihydroxy-octadecenoic acid (DiHOME), dihydroxy-eicosatrienoic acid (diHETrE), isoprostane, and nitro fatty acid eicosanoid families. In conclusion, we did not identify any prostaglandins that would have utility as predictors for term or preterm labour, however, we have identified diverse eicosanoids that have not been previously explored in the context of pregnancy and labour, highlighting novel areas for biomarker research.
类花生酸家族中的前列腺素及其他相关分子长期以来一直被认为与足月分娩和早产过程有关。然而，究竟哪些类花生酸参与其中，以及它们是否可作为分娩的生物标志物，仍有待证实。本研究的目的是确定尿前列腺素及相关分子：a）在足月和早产时是否会随着分娩和/或宫颈变化而改变，和/或b）与先兆早产个体的分娩时间是否相关。将孕妇分为以下几组：n = 32例足月未分娩者，n = 49例足月分娩者，n = 15例早产未分娩对照者，n = 43例先兆早产并早产者，以及n = 44例先兆早产并足月分娩者。通过酶联免疫吸附测定法（ELISA）测量前列腺素PGE2、PGF2α、PGD2和PGI2以及8-异前列腺素的代谢物。此外，在一个小样本（n = 24）子集中，使用基于质谱的靶向脂质组学检测板测量了147种类花生酸。与足月未分娩相比，足月分娩时前列腺素PGF2α、PGE2和PGF2α代谢物增加。分娩开始前，任何前列腺素代谢物均无变化。与足月分娩者相比，先兆早产并早产者的前列腺素I2代谢物较低。在我们的发现队列中，我们确定了另外20种类花生酸在母体尿液中高表达，包括前列腺素、羟基二十碳四烯酸（HETE）、环氧二十碳三烯酸（EET）、二羟基十八碳烯酸（DiHOME）、二羟基二十碳三烯酸（diHETrE）、异前列腺素和硝基脂肪酸类花生酸家族的成员。总之，我们未发现任何可作为足月或早产预测指标的前列腺素，然而，我们确定了多种以前在妊娠和分娩背景下未被探索过的类花生酸，突出了生物标志物研究的新领域。
