Oaks S C, Osterman J V, Hetrick F M
J Clin Microbiol. 1977 Jul;6(1):76-80. doi: 10.1128/jcm.6.1.76-80.1977.
It was demonstrated that gamma-irradiated L-929 cells support plaque formation by three strains of Rickettsia tsutsugamushi and representative species of the spotted fever and typhus group rickettsiae. Sensitivity of the plaque assay for detection of viable scrub typhus rickettsiae was similar to that achieved with intraperitoneal inoculation of random-bred mice. The concentration of irradiated cells and the temperature and length of incubation were all found to affect plaque size. A technique combining terminal dilution and plaque purification was used to obtain clones of three strains of scrub typhus rickettsiae.
结果表明，经γ射线辐照的L-929细胞可支持三株恙虫病立克次体以及斑点热群和斑疹伤寒群立克次体的代表菌株形成噬斑。噬斑试验检测活恙虫病立克次体的敏感性与随机繁殖小鼠腹腔接种法的敏感性相似。研究发现，辐照细胞的浓度、培养温度和培养时间均会影响噬斑大小。采用终末稀释和噬斑纯化相结合的技术获得了三株恙虫病立克次体的克隆。
