Pierce N F, Sack R B
J Infect Dis. 1977 Aug;136 Suppl:S113-7. doi: 10.1093/infdis/136.supplement.s113.
The intestinal immune response to cholera toxoid was studied in dogs and rats. Oral or intraperitoneal priming followed by duodenal boosting with toxoid reulted in antitoxin-containing plasma cells (ACC) in jejunal lamina propria of rats. Priming and boosting by the intraperitoneal route alone induced almost no jejunal response. Lamina propria ACC were derived largely from migrating immunoblasts, which appeared earlier among thoracic duct lymphocytes. Protection of dogs after repeated subcutaneous immunization with toxoid was mediated largely, or entirely, by serum-derived antibody. The sequence of subcutaneous priming and repeated oral boosting induced longer lasting protection mediated almost entirely by the local intestinal immune mechanism. The results suggest that cholera immunization should be specifically designed to stimulate the intestinal immune mechanism and that the subcutaneous oral immunizing sequence may be an efficient way to do this.
在犬类和大鼠中研究了肠道对霍乱类毒素的免疫反应。经口服或腹腔注射进行初次免疫，随后经十二指肠用类毒素加强免疫，可使大鼠空肠固有层中产生含抗毒素的浆细胞（ACC）。仅通过腹腔途径进行初次免疫和加强免疫几乎不会诱导空肠产生反应。固有层ACC主要来源于迁移的免疫母细胞，这些免疫母细胞在胸导管淋巴细胞中更早出现。用类毒素对犬进行反复皮下免疫后的保护作用主要或完全由血清来源的抗体介导。皮下初次免疫和反复口服加强免疫的顺序诱导了更持久的保护作用，这种保护作用几乎完全由局部肠道免疫机制介导。结果表明，霍乱免疫应专门设计以刺激肠道免疫机制，皮下-口服免疫顺序可能是实现这一目的的有效方法。
