Tees R, Schreier M H
Nature. 1980 Feb 21;283(5749):780-1. doi: 10.1038/283780a0.
An antibody response is the end result of complex interactions among T cells, adherent cells and B cells. An understanding of the interactions involved has proved difficult as pure populations of these cells have not been available. By making use of T-cell growth factor, we were able to grow normal helper T cells specific for heterologous erythrocytes. Because specificity and mechanism of action of these cells had been demonstrated solely in culture, we sought to establish their competence in the whole animal. We have therefore examined here whether antigen-specific helper T cells, maintained in culture over long periods, would enable syngeneic nude mice to respond to T-cell dependent antigens. The results show that specific helper T cells, propagated in serum-free medium in vitro for up to 15 months, can selectively and specifically reconstitute syngeneic C57BL/6J nu/nu mice. Depending on the specificity of the injected helper T cells, such nude mice could respond to sheep red blood cells (SRC) but not to horse red blood cells (HRC) and vice versa. The magnitude of the response was comparable to that of normal mice and could exceed it by almost 10-fold, depending on the source and number of injected helper T cells.
抗体反应是T细胞、黏附细胞和B细胞之间复杂相互作用的最终结果。由于无法获得这些细胞的纯群体，已证明了解其中涉及的相互作用很困难。通过利用T细胞生长因子，我们能够培养出对异种红细胞具有特异性的正常辅助性T细胞。由于这些细胞的特异性和作用机制仅在培养中得到证实，我们试图在整体动物中确定它们的功能。因此，我们在此研究了长期在培养中维持的抗原特异性辅助性T细胞是否能使同基因裸鼠对T细胞依赖性抗原作出反应。结果表明，在无血清培养基中体外培养长达15个月的特异性辅助性T细胞，可以选择性地、特异性地重建同基因C57BL/6J nu/nu小鼠的免疫功能。根据注射的辅助性T细胞的特异性，此类裸鼠可对绵羊红细胞（SRC）作出反应，但对马红细胞（HRC）无反应，反之亦然。反应的强度与正常小鼠相当，根据注射的辅助性T细胞的来源和数量，反应强度可能比正常小鼠高出近10倍。
