Segre D, Segre M
J Immunol. 1976 Mar;116(3):735-8.
Mixed cultures of DNP-primed spleen cells from old and young BC3F1 mice in diffusion chambers yielded significantly fewer secondary PFC than expected from the numbers of PFC found when young and old spleen cells were cultured alone. There was a negative correlation between the log of the number of PFC found in cultures of old spleen cells alone and the degree of immunosuppression exerted by the cells. The immunosuppressive effect was abolished by pretreatment of the old spleen cells with anti-theta serum and complement. Thus, the effect was attributed to the presence of large numbers of suppressor T cells in the spleen of primed old mice. No suppression was found in mixed cultures of spleen cells from old unimmunized mice and young primed mice.
将来自老年和幼年BC3F1小鼠的经二硝基苯酚（DNP）致敏的脾细胞混合培养于扩散小室中，产生的继发性空斑形成细胞（PFC）明显少于单独培养幼年和老年脾细胞时根据PFC数量所预期的结果。单独培养老年脾细胞时所发现的PFC数量的对数与这些细胞所施加的免疫抑制程度之间存在负相关。用抗θ血清和补体对老年脾细胞进行预处理可消除免疫抑制作用。因此，这种作用归因于致敏老年小鼠脾脏中存在大量抑制性T细胞。在未免疫的老年小鼠和致敏的幼年小鼠的脾细胞混合培养物中未发现抑制作用。
