Segond P, Delfraissy J F, Galanaud P, Wallon C, Massias P, Dormont J
Clin Exp Immunol. 1979 Aug;37(2):196-204.
We have studied the primary in vitro antibody response toward a hapten in cultures of peripheral blood lymphocytes from twenty-two patients suffering from regular rheumatoid arthritis (RA). These patients were not receiving immunosuppressive drugs or corticosteroids and had not taken Aspirin or non-steroidal anti-inflammatory agents for at least 72 hr. The control groups included thirty-two healthy subjects and twenty-seven control patients. The mean anti-TNP response of the RA patients was significantly lower than that of both control groups. No pre-existing anti-TNP or IgG response could be detected. A search for suppressor cells in co-cultures of RA and normal lymphocytes was negative. On the contrary, the extent of allogeneic enhancement in such co-cultures was comparable to that observed when control lymphocytes were co-cultured. RA serum added to normal lymphocytes cultures showed a dramatic inhibitory effect in only two out of nine cases. A follow-up study has strongly suggested that RA lymphocytes could increase their in vitro antibody response upon treatment.
我们研究了22例患有典型类风湿性关节炎（RA）的患者外周血淋巴细胞培养物中针对半抗原的初次体外抗体反应。这些患者未接受免疫抑制药物或皮质类固醇治疗，且至少72小时未服用阿司匹林或非甾体类抗炎药。对照组包括32名健康受试者和27名对照患者。RA患者的平均抗TNP反应明显低于两个对照组。未检测到预先存在的抗TNP或IgG反应。在RA淋巴细胞与正常淋巴细胞的共培养物中寻找抑制细胞的结果为阴性。相反，这种共培养物中同种异体增强的程度与对照淋巴细胞共培养时观察到的程度相当。添加到正常淋巴细胞培养物中的RA血清仅在9例中的2例中显示出显著的抑制作用。一项随访研究强烈表明，RA淋巴细胞在治疗后其体外抗体反应可能会增强。
