Winterleitner H
Blut. 1980 May;40(5):305-12. doi: 10.1007/BF01025585.
Two of our preparations of rabbit immune sera against Non-T, Non-B ALL cells were characterized. After a previously described absorption schedule used at present with some modifications, the sera were tested by indirect immunofluorescence and by a complement-dependent microcytotoxicity technique on a panel of cells: various types of ALL, lymphoblastoid cell lines of T, B and Non-T, Non-B surface characteristics, other leukemias (AML, CLL, Ph1 positive CML-BC), nonleukemic tissue of normal bone marrow, lymph-nodes and tonsils and also PBL. About 70% of Non-T, Non-B ALL, the Non-T, Non-B ALL line "REH" and CML cells in Ph1 positive "lymphoid" blast crisis were reactive in both test systems. Non-regenerating normal bone marrow, peripheral blood lymphocytes, and lymph node cells were non-reactive. CLL cells and tonsil cels, however, showed a positive fluorescence, yet gave a negative reaction in the complement-dependent microcytotoxicity test. This phenomenon might be explained by unspecific binding to Fc receptors. The antisera thus appear to detect a common Non-T, Non-B ALL antigen.
我们制备的两种抗非T、非B急性淋巴细胞白血病（ALL）细胞的兔免疫血清得到了鉴定。按照目前使用的并经一些修改的先前描述的吸收方案处理后，通过间接免疫荧光法以及补体依赖的微量细胞毒性技术，在一组细胞上对血清进行了检测：各种类型的ALL、具有T、B以及非T、非B表面特征的淋巴母细胞系、其他白血病（急性髓系白血病、慢性淋巴细胞白血病、Ph1阳性慢性髓系白血病急变期）、正常骨髓、淋巴结和扁桃体的非白血病组织以及外周血淋巴细胞。在这两种检测系统中，约70%的非T、非B ALL、非T、非B ALL细胞系“REH”以及Ph1阳性“淋巴样”急变期的慢性髓系白血病细胞呈反应性。未再生的正常骨髓、外周血淋巴细胞和淋巴结细胞无反应性。然而，慢性淋巴细胞白血病细胞和扁桃体细胞显示出阳性荧光，但在补体依赖的微量细胞毒性试验中呈阴性反应。这种现象可能是由于与Fc受体的非特异性结合所致。因此，这些抗血清似乎检测到了一种共同的非T、非B ALL抗原。
