Naumenko E V, Shishkina G T
Endocrinol Exp. 1980 Sep;14(3):207-13.
Complete deafferentation of mediobasal hypothalamus did not prevent a decrease of testosterone level in plasma of male rats after a hemicastration. From this finding a conclusion on a relative autonomy of hypophysiotropic area of hypothalamus in regulating the blood testosterone level by a negative feed-back mechanism was drawn. However, the administration of serotonin (200 micrograms in 10 microliters) into the lateral ventricle in rat males bearing a complete deafferentation of mediobasal hypothalamus resulted in a blocking of compensatory increase of testosterone level in plasma after a hemicastration as compared to either intact or just deafferented animals in which the level of testosterone reached the initial value at 72 h after a hemicastration. Finally, the experiments with the injections of serotonin (2.5 micrograms in 2 microliters) into various areas of mediobasal hypothalamus showed that the site of inhibitory effect of stimulated serotoninergic receptors on the mechanism of negative feed-back in pituitary-testicular system may be located in arcuate nuclei.
完全切断下丘脑内侧基底部的传入神经，并未阻止雄性大鼠半侧睾丸切除后血浆睾酮水平的下降。基于这一发现，得出了关于下丘脑促垂体区在通过负反馈机制调节血液睾酮水平方面具有相对自主性的结论。然而，向完全切断下丘脑内侧基底部传入神经的雄性大鼠侧脑室内注射血清素（10微升含200微克），与完整或仅去传入神经的动物相比，半侧睾丸切除后血浆睾酮水平的代偿性升高受到了阻断，在完整或仅去传入神经的动物中，睾酮水平在半侧睾丸切除后72小时达到初始值。最后，向内侧基底部下丘脑的不同区域注射血清素（2微升含2.5微克）的实验表明，受刺激的血清素能受体对垂体 - 睾丸系统负反馈机制的抑制作用位点可能位于弓状核。
