Romagnani S, Maggi E, Del Prete G, Biti G, Ponticelli P, Ricci M
Cancer. 1980 Dec 15;46(12):2590-5. doi: 10.1002/1097-0142(19801215)46:12<2590::aid-cncr2820461210>3.0.co;2-d.
The present study was undertaken to determine the effect of radiotherapy on subpopulations of peripheral blood T cells from patients with Hodgkin's disease. T cell were purified from each specimen, and proportions and absolute numbers of T lymphocytes bearing receptors for the Fc portion of IgG (TG) and for Fc portion of IgM (TM) were enumerated by rosetting T cells with ox red blood cells (ORBC), which had been coated with anti-ORBC rabbit IgG or IgM, respectively. In untreated patients, the percentage of TG cells was significantly increased, and the percentage of TM cells was significantly decreased when compared with control values. In patients examined after radiotherapy, there was a severe depletion of total T lymphocytes. The percent and absolute values of TM cells were also markedly decreased in comparison with those found in either normal controls or untreated patients. In contrast, relative proportions of TG cells were significantly increased in the same treated patients, but the absolute numbers of these cells were essentially unchanged in comparison with those found before radiation therapy. There was a partial and progressive restoration of the number of TM lymphocytes some years after the treatment, but reduced percentages of TM lymphocytes and increased percentages of TG lymphocytes were still found in patients in continuous complete remission for at least five years or more. Similar alterations of the two T-cell subsets were also found in the peripheral blood of a small group of patients treated with radiation for testicular seminoma. These data clearly demonstrate that radiation therapy has a differential effect on identifiable and distinct subsets of cells in the human T-cell class.
本研究旨在确定放射治疗对霍奇金病患者外周血T细胞亚群的影响。从每个标本中纯化T细胞，通过用分别包被有抗ORBC兔IgG或IgM的氧化红细胞（ORBC）对T细胞进行玫瑰花结试验，来计数带有IgG Fc段受体（TG）和IgM Fc段受体（TM）的T淋巴细胞的比例和绝对数量。在未经治疗的患者中，与对照值相比，TG细胞百分比显著增加，TM细胞百分比显著降低。在放疗后检查的患者中，总T淋巴细胞严重耗竭。与正常对照或未经治疗的患者相比，TM细胞的百分比和绝对值也显著降低。相比之下，在同一治疗患者中，TG细胞的相对比例显著增加，但与放疗前相比，这些细胞的绝对数量基本未变。治疗后数年间，TM淋巴细胞数量有部分且逐渐的恢复，但在持续完全缓解至少五年或更长时间的患者中，仍发现TM淋巴细胞百分比降低，TG淋巴细胞百分比增加。在一小群接受睾丸精原细胞瘤放疗的患者外周血中也发现了这两个T细胞亚群的类似改变。这些数据清楚地表明，放射治疗对人类T细胞类中可识别和不同的细胞亚群有不同的影响。
