Price G B, Senn J S, McCulloch E A, Till J E
Biochem J. 1975 May;148(2):209-17. doi: 10.1042/bj1480209.
The colony-stimulating activity detected by its ability to promote colony formation by human granulopoietic progenitor cells was partially purified from medium conditioned by human peripheral leucocytes. The purification procedure utilized (NH4)2SO4 precipitation, DEAE-cellulose chromatography, hydroxyapatite chromatography and gel filtration on Sephadex G-150 and yielded a purification of about 1000-fold. The medium from cultures of non-leukaemic cells contained three molecular species of colony-stimulating activity with approximate molecular weights of 93000, 36500 and 14700. On the basis of their sensitivity to enzymes, these species of activity appeared to be proteins. In contrast, medium from cultures of leukaemic cells contained only one detectable molecular species with colony-stimulating activity, usually with an approximate molecular weight of 36500. The results are discussed in relation to concurrent studies on the association of the different species of colony-stimulating activity with the cell surface membrane.
通过人粒细胞祖细胞促进集落形成的能力检测到的集落刺激活性，从人外周血白细胞条件培养基中部分纯化。纯化过程采用硫酸铵沉淀、DEAE-纤维素色谱、羟基磷灰石色谱以及Sephadex G-150凝胶过滤，得到了约1000倍的纯化倍数。非白血病细胞培养物的培养基中含有三种集落刺激活性分子种类，分子量约为93000、36500和14700。基于它们对酶的敏感性，这些活性种类似乎是蛋白质。相比之下，白血病细胞培养物的培养基中仅含有一种可检测到的具有集落刺激活性的分子种类，通常分子量约为36500。结合关于不同种类集落刺激活性与细胞表面膜关联的同期研究对结果进行了讨论。
