Antel J, Oger J J, Jackevicius S, Kuo H H, Arnason B G
Proc Natl Acad Sci U S A. 1982 May;79(10):3330-4. doi: 10.1073/pnas.79.10.3330.
Effects of the anti-T-cell monoclonal antibodies OKT3, OKT5, and OKT8 on T-cell surface properties and cell functions were evaluated. Incubation of mononuclear cells isolated from peripheral blood for 48 hr with each monoclonal antibody in the absence of complement resulted in modulation of their respective surface antigens; i.e., the number of cells detected by immunofluorescence as positive for the T3, T5, and T8 surface antigens was reduced. T3, T5, and T8 antigens modulated independently. A radiolabeled second antibody technique confirmed modulation by OKT3 and OKT8 and indicated that T-cell differentiation antigens can regenerate in culture. Incubation of mononuclear cells with OKT3 increased the number of sheep erythrocyte-binding lymphocytes (E+-rosetting cells) and markedly increased the number of avidly E+-rosetting cells. Incubation with OKT8 reduced the number of E+- and of avidly E+-rosetting cells. OKT3 induced both mitogenic reactivity and suppressor cell activity; cells modulated by OKT8 exhibited reduced mitogenic reactivity and reduced suppressor cell function. The decreases in total T cells, in avid T cells, in suppressor cell number, and in suppressor cell function that follow modulation by OKT8 mimic changes observed in multiple sclerosis patients.
评估了抗T细胞单克隆抗体OKT3、OKT5和OKT8对T细胞表面特性和细胞功能的影响。在无补体的情况下，将从外周血分离的单核细胞与每种单克隆抗体孵育48小时，导致其各自表面抗原的调节；即，通过免疫荧光检测为T3、T5和T8表面抗原阳性的细胞数量减少。T3、T5和T8抗原独立调节。放射性标记的二抗技术证实了OKT3和OKT8的调节作用，并表明T细胞分化抗原可在培养中再生。用OKT3孵育单核细胞增加了绵羊红细胞结合淋巴细胞（E+花环形成细胞）的数量，并显著增加了强亲和性E+花环形成细胞的数量。用OKT8孵育减少了E+和强亲和性E+花环形成细胞的数量。OKT3诱导有丝分裂反应性和抑制细胞活性；经OKT8调节的细胞表现出降低的有丝分裂反应性和降低的抑制细胞功能。OKT8调节后总T细胞、活化T细胞、抑制细胞数量和抑制细胞功能的减少模拟了在多发性硬化症患者中观察到的变化。
