Cicciarelli J C, Chia D, Terasaki P I, Barnett E V, Shirahama S
Tissue Antigens. 1980 Mar;15(3):275-82. doi: 10.1111/j.1399-0039.1980.tb00918.x.
B lymphocytes were shown previously to be killed at 5 degrees C by some autologous and allogeneic human sera. We show here that such cold cytotoxins are directed against immunoglobulins on the surface of B lymphocytes. The activity is partially removed by passing serum through IgG-coupled Sepharose and usually completely removed by passing serum through IgM-coupled Sepharose. Activity is regained from the columns by acid elution and IgM inhibits the cytotoxicity of these eluates. 125I-labeled eluates bind to IgG- and IgM-coupled Sepharose beads and binding is inhibited by IgM and to a lesser degree by IgG thus showing a primarily more avid binding to IgM as compared to IgG. Furthermore, the 125I-labeled cytotoxic eluates recognize the same determinant(s) on IgM and IgG. We suggest tht IgM anti-IgM antiimmunoglobulin may regulate immune reactivity by binding to B-lymphocyte surfaces.
先前已表明，一些自体和同种异体人血清在5摄氏度时可杀死B淋巴细胞。我们在此表明，此类冷细胞毒素针对的是B淋巴细胞表面的免疫球蛋白。通过使血清通过IgG偶联的琼脂糖凝胶，活性可部分去除，而通常通过使血清通过IgM偶联的琼脂糖凝胶，活性可完全去除。通过酸洗脱可从柱上恢复活性，并且IgM可抑制这些洗脱液的细胞毒性。125I标记的洗脱液与IgG和IgM偶联的琼脂糖珠结合，并且结合受到IgM的抑制，而受到IgG的抑制程度较小，因此与IgG相比，显示出对IgM的结合亲和力更高。此外，125I标记的细胞毒性洗脱液识别IgM和IgG上的相同决定簇。我们认为，IgM抗IgM抗免疫球蛋白可能通过与B淋巴细胞表面结合来调节免疫反应性。
