Distelhorst C W, Benutto B M
J Immunol. 1981 Apr;126(4):1630-4.
Glucocorticoid receptors were measured in T lymphocytes that were isolated from peripheral blood by either nylon wool filtration or E-rosette sedimentation. T cells isolated by nylon wool filtration specifically bind 6.7 +/- 0.2 fmol of dexamethasone per million cells (equivalent to 4000 +/- 200 receptors per cell), whereas T cells isolated by E-rosette sedimentation bind 12.0 +/- 0.7 fmol of dexamethasone per million cells (equivalent to 7200 +/- 400 receptors per cell). This difference in the amount of dexamethasone bound by the two T cell preparations was significant (p less than .001) and was present immediately after cell isolation. The binding affinities of the different T cell preparations for dexamethasone were similar. T cells that are isolated by a combination of nylon wool filtration followed by E-rosette sedimentation bind the same amount of dexamethasone as T cells isolated by nylon wool filtration alone. T cells isolated by a combination of E-rosette sedimentation following by nylon wool filtration bind less dexamethasone than do T cells isolated by E-rosette sedimentation alone. These findings suggest that T cells are heterogeneous with respect to their quantity of glucocorticoid receptors. Isolation of T cells by E-rosette sedimentation enriches for T cells that have a greater number of glucocorticoid receptors, and isolation of T cells by nylon wool filtration enriches for T cells that have a lesser number of glucocorticoid receptors.
通过尼龙毛过滤或E花环沉降法从外周血中分离出的T淋巴细胞中检测了糖皮质激素受体。通过尼龙毛过滤分离出的T细胞每百万细胞特异性结合6.7±0.2飞摩尔地塞米松（相当于每细胞4000±200个受体），而通过E花环沉降法分离出的T细胞每百万细胞结合12.0±0.7飞摩尔地塞米松（相当于每细胞7200±400个受体）。两种T细胞制剂结合的地塞米松量的这种差异具有显著性（p<0.001），并且在细胞分离后立即就存在。不同T细胞制剂与地塞米松的结合亲和力相似。通过尼龙毛过滤后再进行E花环沉降法联合分离出的T细胞与仅通过尼龙毛过滤分离出的T细胞结合相同量的地塞米松。通过E花环沉降法后再进行尼龙毛过滤联合分离出的T细胞比仅通过E花环沉降法分离出的T细胞结合的地塞米松更少。这些发现表明，T细胞在糖皮质激素受体数量方面是异质性的。通过E花环沉降法分离T细胞可富集具有更多糖皮质激素受体的T细胞，而通过尼龙毛过滤法分离T细胞可富集具有较少糖皮质激素受体的T细胞。
