Lippman M, Barr R
J Immunol. 1977 Jun;118(6):1977-81.
On the premise that the differential effects of glucocorticoids on various aspects of the immune response may be mediated by differences in the glucocorticoid receptors in the effector cells, subpopulations of human peripheral blood lymphocytes were examined for these receptors as well as for glucocorticoid responsiveness. Purified T and non-T lymphocytes, when studied by a sensitive whole cell assay technique, contained equivalent amounts of specific glucocorticoid receptor, which, by binding affinity and specificity measurements, were indistinguishable from each other. Furthermore, under in vitro incubation conditions, macromolecular synthesis in both of these cell populations was inhibited by glucocorticoid at concentrations which saturated the receptor sites. It is concluded that the putative differential effects of glucocorticoids on T and non-T lymphocyte-associated functions are probably not mediated by differences in the glucocorticoid receptors in these cell populations.
基于糖皮质激素对免疫反应各个方面的不同作用可能是由效应细胞中糖皮质激素受体的差异所介导的这一前提，对人外周血淋巴细胞亚群进行了这些受体以及糖皮质激素反应性的检测。当通过灵敏的全细胞检测技术进行研究时，纯化的T淋巴细胞和非T淋巴细胞含有等量的特异性糖皮质激素受体，通过结合亲和力和特异性测量，它们彼此无法区分。此外，在体外培养条件下，这两种细胞群体中的大分子合成在使受体位点饱和的糖皮质激素浓度下受到抑制。得出的结论是，糖皮质激素对T淋巴细胞和非T淋巴细胞相关功能的假定不同作用可能不是由这些细胞群体中糖皮质激素受体的差异所介导的。
