Wong L G, Colburn K K, Kacena A, Weisbart R H
Immunopharmacology. 1981 Jun;3(2):179-85. doi: 10.1016/0162-3109(81)90018-7.
Glucocorticoids may suppress cell-mediated immunity by inhibiting lymphocyte mediator production or reducing the responsiveness of target cells to these mediators. Our laboratory recently described a newly recognized T-lymphocyte mediator, neutrophil migration inhibition factor from T-lymphocytes (NIF-T). In this report we assessed the effect of glucocorticoids on NIF-T activity. Methylprednisolone (MP) at concentrations as low as 10-7 M inhibited NIF-T activity from peripheral blood lymphocytes (PBL) in response to staphylococcal protein A (SPA) and concanavalin A (Con A). However, MP at concentrations as high as 10-4 M did not after the responsiveness of neutrophils to NIF-T. Therefore, the effect of MP on NIF-T activity was due to inhibition of mediator production. The effect of MP on NIF-T production was reversible in 24 hours. This finding is consistent with the clinical observation that alternate day therapy does not suppress cell-mediated immunity. Serum taken from a patient as early as one hour after oral administration of 100 mg of prednisone inhibited NIF-T production in vitro; serum obtained at 48 hr after prednisone had no measurable effect on NIF-T activity, In addition. MP inhibited NIF-T production by previously activated lymphocytes.
糖皮质激素可通过抑制淋巴细胞介质的产生或降低靶细胞对这些介质的反应性来抑制细胞介导的免疫。我们实验室最近描述了一种新发现的T淋巴细胞介质，即来自T淋巴细胞的中性粒细胞迁移抑制因子（NIF-T）。在本报告中，我们评估了糖皮质激素对NIF-T活性的影响。浓度低至10^-7 M的甲基泼尼松龙（MP）可抑制外周血淋巴细胞（PBL）对葡萄球菌蛋白A（SPA）和刀豆球蛋白A（Con A）产生的NIF-T活性。然而，浓度高达10^-4 M的MP并未改变中性粒细胞对NIF-T的反应性。因此，MP对NIF-T活性的影响是由于抑制了介质的产生。MP对NIF-T产生的影响在24小时内是可逆的。这一发现与隔日疗法不会抑制细胞介导免疫的临床观察结果一致。早在口服100 mg泼尼松后1小时从患者采集的血清可在体外抑制NIF-T的产生；在泼尼松给药后48小时获得的血清对NIF-T活性没有可测量的影响。此外，MP可抑制先前活化的淋巴细胞产生NIF-T。
