Hill B C, Courtney K R
Biophys J. 1982 Dec;40(3):255-7. doi: 10.1016/S0006-3495(82)84481-0.
Muscle contraction can introduce artifact in attempted optical measurements of the action potential in heart tissue stained with voltage-sensitive dyes. Using rabbit sinus node and atrial tissue in vitro, we found that the voltage-sensitive part of the optical signal remains relatively unchanged by variations in the rate of external stimulation or by the application of transmural stimulation (TS), while the contraction-related component can be significantly increased by these same interventions. The relative contributions of membrane voltage and contraction to the optical signal can thus be determined. In particular, the rapid upstroke component of the action potential can be easily identified using this technique.
肌肉收缩会在试图对用电压敏感染料染色的心脏组织动作电位进行光学测量时引入伪迹。我们使用体外培养的兔窦房结和心房组织发现，光学信号的电压敏感部分在外部刺激速率变化或施加透壁刺激（TS）时相对保持不变，而与收缩相关的成分可通过这些相同干预显著增加。因此，可以确定膜电压和收缩对光学信号的相对贡献。特别是，使用该技术可以很容易地识别动作电位的快速上升成分。
