Hafiz S, Kinghorn G R, McEntegart M G
Br J Vener Dis. 1981 Dec;57(6):382-6. doi: 10.1136/sti.57.6.382.
The causative organism of chancroid, Haemophilus ducreyi, is generally considered to be very fastidious and its isolation, maintenance, and detailed study very demanding. In this study a modified medium was developed, which allowed the organism to be isolated more frequently than previously would have been expected. Twenty-two cases of chancroid were confirmed by the isolation of H ducreyi in 160 patients with genital ulceration examined over a one-year period. The cases were apparently unrelated, and in only five was there a history of recent sexual contact abroad. Concurrent infection with other sexually transmitted diseases was present in 18 (81.8%) patients, and in 14 (63.6%) both H ducreyi and herpes simplex virus were isolated form the same genital ulcers. Thus, these findings indicate that chancroid is underdiagnosed in England and that H ducreyi may frequently occur as a secondary invader of damaged genital skin and mucosa.
软下疳的病原体杜克雷嗜血杆菌通常被认为是一种苛求菌，其分离、培养及详细研究都极具挑战性。在本研究中，开发了一种改良培养基，使得该菌的分离频率高于以往预期。在为期一年的时间里，对160例生殖器溃疡患者进行检查，通过分离出杜克雷嗜血杆菌确诊了22例软下疳病例。这些病例显然无关联，只有5例有近期国外性接触史。18例（81.8%）患者同时感染了其他性传播疾病，14例（63.6%）患者的同一生殖器溃疡处同时分离出了杜克雷嗜血杆菌和单纯疱疹病毒。因此，这些发现表明软下疳在英国未得到充分诊断，且杜克雷嗜血杆菌可能经常作为受损生殖器皮肤和黏膜的继发性入侵者出现。
