Jones B M, Hafiz S, Duerden B I
Antimicrob Agents Chemother. 1986 Jun;29(6):1110-2. doi: 10.1128/AAC.29.6.1110.
The MICs of ampicillin, ampicillin plus sulbactam in equal proportions, and a range of ampicillin concentrations with a constant 0.5 micrograms/ml concentration of sulbactam were determined for 66 strains of Haemophilus ducreyi by an agar dilution technique with standardized inocula prepared by ultrasonication. Fifty-five strains were susceptible to ampicillin alone (MIC range, 0.06 to 1 microgram/ml). The MICs for the 11 resistant strains were (micrograms per milliliter): range, 8 to greater than 16; MIC for 50% of the strains tested (MIC50), greater than 16; MIC90, greater than 16; 8 of them produced beta-lactamase. In the presence of an equal concentration of sulbactam, the MICs for ampicillin-resistant strains were lowered to (micrograms per milliliter): range, 0.125 to 2; MIC50, 0.25; MIC90, 1. In the presence of a fixed 0.5-micrograms/ml concentration of sulbactam, the MICs for the resistant strains were (micrograms per milliliter): range, 0.125 to 2; MIC50, 0.125; MIC90, 0.25. Sulbactam-ampicillin appears to be suitable for the treatment of H. ducreyi infections, especially those caused by ampicillin-resistant strains.
采用琼脂稀释法，通过超声处理制备标准化接种物，测定了66株杜克雷嗜血杆菌对氨苄西林、等比例的氨苄西林加舒巴坦以及一系列氨苄西林浓度与固定浓度0.5微克/毫升舒巴坦的最低抑菌浓度（MIC）。55株菌株对单独的氨苄西林敏感（MIC范围为0.06至1微克/毫升）。11株耐药菌株的MIC（微克/毫升）分别为：范围8至大于16；50%受试菌株的MIC（MIC50）大于16；MIC90大于16；其中8株产生β-内酰胺酶。在舒巴坦浓度相等的情况下，耐氨苄西林菌株的MIC降至（微克/毫升）：范围0.125至2；MIC50为0.25；MIC90为1。在舒巴坦固定浓度为0.5微克/毫升的情况下，耐药菌株的MIC（微克/毫升）分别为：范围0.125至2；MIC50为0.125；MIC90为0.25。舒巴坦-氨苄西林似乎适用于治疗杜克雷嗜血杆菌感染，尤其是由耐氨苄西林菌株引起的感染。
