Cunningham-Rundles C
J Exp Med. 1982 Mar 1;155(3):711-9. doi: 10.1084/jem.155.3.711.
50% of individuals of selective IgA deficiency have high serum titers of antibody to bovine proteins, and high levels of circulating immune complexes that contain bovine antigens. Because in animal studies, immunization with antigen-antibody complexes is a very effective means of producing anti-idiotypic antibodies, we sought such autoantibodies in two sera known to have large amounts of anticasein. After IgG isolation and two-stage affinity chromatography, IgG-like material (molecular weights of H and L chains on SDS-PAGE), with binding activity for the F(ab')2 of anticasein were isolated from both sera. Pooled human gamma globulin or IgG myeloma proteins did not inhibit binding of specific anti-anticaseins to the corresponding anticasein, but sodium caseinate did block this binding (by 80 and 95%) indicating that most of these autoantibodies have affinity for the casein-binding site. Naturally occurring anti-idiotypic antibodies have been difficult to conclusively demonstrate in human sera; consequently, these experiments provide evidence of a unique model which may be used to explore the network theory of immunoglobulin regulation in humans.
选择性IgA缺乏症患者中有50%血清中抗牛蛋白抗体滴度高，且含有牛抗原的循环免疫复合物水平高。因为在动物研究中，用抗原-抗体复合物免疫是产生抗独特型抗体的一种非常有效的方法，所以我们在两份已知含有大量抗酪蛋白抗体的血清中寻找此类自身抗体。在分离IgG并经过两阶段亲和层析后，从两份血清中均分离出了具有IgG样特征的物质（SDS-PAGE上重链和轻链的分子量），其对抗酪蛋白的F(ab')2具有结合活性。混合的人γ球蛋白或IgG骨髓瘤蛋白并不抑制特异性抗抗酪蛋白抗体与相应抗酪蛋白的结合，但酪蛋白酸钠确实能阻断这种结合（分别为80%和95%），这表明这些自身抗体中的大多数对酪蛋白结合位点具有亲和力。天然存在的抗独特型抗体很难在人血清中得到确凿证明；因此，这些实验提供了一个独特模型的证据，该模型可用于探索人类免疫球蛋白调节的网络理论。
