Pasquali J L, Fong S, Tsoukas C, Vaughan J H, Carson D A
J Clin Invest. 1980 Oct;66(4):863-6. doi: 10.1172/JCI109927.
The idiotypic determinants on IgM rheumatoid factor (RF) from a single family have been analyzed. Rabbit Fab'2 antiidiotypic antibody was prepared against purified IgM-RF from a patient with rheumatoid arthritis. As measured by radioimmunoassay, the antiidiotype reacted with at least 90% of the patient's RF, but not with non-RF immunoglobulins from the same serum, nor with 10 of 11 polyclonal and monoclonal RF from unrelated individuals. Cross-reacting idiotypes were detected on RF in four of the patients' first degree relatives, spanning three generations, without apparent relation of HLA type or clinical rheumatoid arthritis. These results suggest that IgM-RF associated idiotypes were inherited in this family.
对来自一个家族的IgM类风湿因子（RF）的独特型决定簇进行了分析。用来自一名类风湿性关节炎患者纯化的IgM-RF制备兔Fab'2抗独特型抗体。通过放射免疫测定法测定，该抗独特型抗体与至少90%的患者RF发生反应，但不与来自同一血清的非RF免疫球蛋白反应，也不与来自无关个体的11种多克隆和单克隆RF中的10种反应。在三代患者的四名一级亲属的RF上检测到交叉反应独特型，与HLA类型或临床类风湿性关节炎无明显关联。这些结果表明，该家族中IgM-RF相关独特型是可遗传的。
