Milon G, Marchal G, Seman M, Truffa-Bachi P
Agents Actions. 1981 Dec;11(6-7):612-4. doi: 10.1007/BF01978763.
In vivo primed T cells injected into the footpad of naïve recipients elicit a typical delayed-type hypersensitivity (DTH) reaction in the presence of their specific antigen. Serial dilutions of primed T cell populations were used in order to score positive and negative transfers. The values for the footpad swelling obtained after these transfers followed a bimodal distribution. This clear bimodal distribution with no overlapping between positive and negative transfers suggested that a single cell initiates the DTH reaction. Limiting dilutions of cloned T cells transferred with their antigen allowed us to demonstrate that a single cell is able to transfer a specific DTH reaction.
