细胞毒性T淋巴细胞对转移的主要组织相容性复合体基因产物的特异性识别。
Specific recognition of the product of a transferred major histocompatibility complex gene by cytotoxic T lymphocytes.
作者信息
Woodward J G, Orn A, Harmon R C, Goodenow R S, Hood L, Frelinger J A
出版信息
Proc Natl Acad Sci U S A. 1982 Jun;79(11):3613-7. doi: 10.1073/pnas.79.11.3613.
Abstract
Mouse L cells transfected with a genomic clone containing the H-2Ld gene (8-5 cells) were shown to function as targets for H-2Ld-specific cytotoxic T lymphocytes (CTL). The CTL-mediated lysis of 8-5 cells was shown to be H-2Ld specific by the use of (i) CTL with restricted reactivity, (ii) unlabeled target inhibiton, and (iii) monoclonal antibody inhibiton. We also demonstrated that 8-5 cells could function as targets for antibody-plus-complement-mediated cell lysis. Specificity was confirmed by using H-2Ld-specific monoclonal antibodies. These experiments demonstrate that the gene products of a major histocompatibility complex genomic clone can be functionally expressed in a foreign cell and can mediate immunologically specific cellular interactions.
摘要
用含有H-2Ld基因的基因组克隆转染的小鼠L细胞（8-5细胞）被证明可作为H-2Ld特异性细胞毒性T淋巴细胞（CTL）的靶细胞。通过以下方法表明CTL介导的8-5细胞裂解具有H-2Ld特异性：（i）反应受限的CTL，（ii）未标记的靶细胞抑制，以及（iii）单克隆抗体抑制。我们还证明8-5细胞可作为抗体加补体介导的细胞裂解的靶细胞。使用H-2Ld特异性单克隆抗体证实了特异性。这些实验表明，主要组织相容性复合体基因组克隆的基因产物可以在异源细胞中功能性表达，并可介导免疫特异性细胞相互作用。
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