Veien N K, Svejgaard E
Br J Dermatol. 1978 Aug;99(2):191-6. doi: 10.1111/j.1365-2133.1978.tb01981.x.
A lymhocyte transformation test with cobalt chloride was performed in 14 patients who had positive cobalt patch tests and eczema compatible with a cobalt induced contact dermatitis and in 9 controls. Eight of the patients had strongly positive patch tests, while 6 had weakly positive patch tests. Patients and controls showed equivalent responses to the mitogens, phytohaemagglutinin, pokeweed mitogen, and concanavalin A, as well as to the recall antigens, purified protein derivative and Candida albicans. A 1:5000 dilution in RPMI-1640 medium of a 1% aqueous solution of cobalt chloride proved optimal in distinguishing patients from controls. With this dilution, a significantly higher lymphocyte transformation was found in patients with strongly positive cobalt patch tests than in the controls. No significant difference was detected when the results for patients with weakly positive patch tests were compared with those of the controls.
对14名钴斑贴试验呈阳性且患有与钴诱导的接触性皮炎相符的湿疹的患者以及9名对照者进行了氯化钴淋巴细胞转化试验。其中8名患者斑贴试验呈强阳性，6名呈弱阳性。患者和对照者对丝裂原植物血凝素、商陆丝裂原和刀豆球蛋白A以及回忆抗原纯化蛋白衍生物和白色念珠菌的反应相当。事实证明，将1%氯化钴水溶液在RPMI-1640培养基中按1:5000稀释，最有利于区分患者和对照者。采用这种稀释度时，钴斑贴试验呈强阳性的患者的淋巴细胞转化率显著高于对照者。弱阳性斑贴试验患者的结果与对照者相比未发现显著差异。
