Arnadottir T, Kekomäki R, Lund G A, Reunanen M, Salmi A A
J Neurol Sci. 1982 Sep;55(3):273-83. doi: 10.1016/0022-510x(82)90126-5.
Two hundred and twenty-eight paired serum and CSF samples collected from 31 patients with MS during a 2-3-year follow-up were analyzed for the presence of immune complexes (IC) by C1q RIA and PIPA (platelet [125I]protein A) techniques. One hundred and forty-four sera from 11 healthy individuals were analyzed as controls. In almost all patients (29/31) IC were detectable during some period of the disease, as tested by either of the techniques. The results obtained by C1q RIA and PIPA correlated positively with each other in MS when mean serum values of each patient were compared. The mean CSF IC levels detected by C1q RIA appeared to correlate to the mean IgG indexes, an indicator of the intrathecal rate of IgG synthesis. The amount of IC in serum and CSF fluctuated independently in MS. The results of the PIPA test for MS serum IC correlated significantly to the duration of the disease. The PIPA test results also showed that patients in stable or chronic phases of MS displayed IC in serum and CSF more often than patients with a relapsing/remitting course of disease but there was no clear correlation between fluctuations in IC levels in individual patients measured by C1q RIA or PIPA techniques and the disease course. Because of the lack of a clear correlation between the presence, quantity and fluctuation of IC and the clinical picture we suggest that those IC detected in the present study are probably not a precipitating factor in the pathogenesis of multiple sclerosis.
对31例多发性硬化症（MS）患者在2至3年随访期间采集的228对血清和脑脊液样本，采用C1q放射免疫分析（RIA）和血小板[125I]蛋白A（PIPA）技术分析免疫复合物（IC）的存在情况。将11名健康个体的144份血清作为对照进行分析。通过这两种技术中的任何一种检测发现，几乎所有患者（29/31）在疾病的某些阶段均可检测到IC。在MS患者中，当比较每位患者的平均血清值时，C1q RIA和PIPA获得的结果呈正相关。通过C1q RIA检测到的脑脊液IC平均水平似乎与平均IgG指数相关，IgG指数是鞘内IgG合成率的一个指标。在MS患者中，血清和脑脊液中IC的量独立波动。MS血清IC的PIPA检测结果与疾病持续时间显著相关。PIPA检测结果还显示，处于MS稳定期或慢性期的患者血清和脑脊液中出现IC的频率高于疾病呈复发/缓解病程的患者，但通过C1q RIA或PIPA技术测量的个体患者IC水平波动与疾病病程之间没有明显相关性。由于IC的存在、数量和波动与临床表现之间缺乏明确的相关性，我们认为本研究中检测到的那些IC可能不是多发性硬化症发病机制中的促发因素。
