Fauser A A, Neumann H A, Bross K G, Kanz L, Löhr G W
Blood. 1982 Dec;60(6):1317-20.
Pluripotent stem cells (CFU-GEMM) give rise to multilineage hemopoietic colonies in culture. The cellular composition revealed that mixed colonies contain cells of different myeloid lineages and mononuclear cells with T-cell surface antigens. T lymphocytes of primary colonies and replated secondary clones from 5 patients with Hodgkin's lymphoma (stage I--II) were identified by their reaction with the monoclonal antibody OKT-8. Replated secondary clones do act functionally as cytotoxic cells using K562 as target cells. Evidence for a common progenitor of myeloid and lymphoid cells is provided by analysis of individual secondary colonies with the use of OKT-3, OKT-4, OKT-8, VIM-D5, and IgM + D antibodies for each individual clone. Primary mixed and replated secondary colonies revealed OKT-8-positive cells. No reaction with OKT-3, OKT-4, VIM-D 5, or IgM + D was observed. In mixed colonies grown from putative bone marrow transplant donors, only OKT-3-positive cells could be observed. Secondary replated colonies did not stain for OKT-8 and failed to lyse 51Cr-labeled K562 cells.
多能干细胞（CFU-GEMM）在培养中可产生多谱系造血集落。细胞组成显示，混合集落包含不同髓系谱系的细胞以及具有T细胞表面抗原的单核细胞。通过与单克隆抗体OKT-8反应，鉴定了5例霍奇金淋巴瘤（I-II期）患者原代集落和再接种的二代克隆中的T淋巴细胞。以K562为靶细胞时，再接种的二代克隆确实发挥细胞毒性细胞的功能。通过对每个单独克隆使用OKT-3、OKT-4、OKT-8、VIM-D5和IgM + D抗体分析单个二代集落，为髓系和淋巴系细胞的共同祖细胞提供了证据。原代混合集落和再接种的二代集落显示有OKT-8阳性细胞。未观察到与OKT-3、OKT-4、VIM-D5或IgM + D的反应。在来自假定骨髓移植供体的混合集落中，仅可观察到OKT-3阳性细胞。再接种的二代集落未被OKT-8染色，也未能裂解51Cr标记的K562细胞。
