Laham M N, Caldwell J R, Panush R S
J Clin Lab Immunol. 1982 Oct;9(1):39-47.
Recent data have shown a definite relationship between complement and lymphocytes. Genetic studies have demonstrated close linkage between the genes controlling synthesis of complement components C4 and C2 and the major histocompatibility complex. Working in a complement-free environment, we studied the effect of purified human complement components, individually and in various combinations, upon in vitro proliferative responses of human lymphocytes to mitogens and antigens. It was found that the early complement components C1, C4 and C2, together, modulate lymphocyte responses to these various stimuli. In general, doses up to 1,000 effective molecules of each component per lymphocyte enhanced the cells' responses to both mitogens and antigens. Higher doses, up to 3,000 effective molecules of each per lymphocyte, progressively inhibited the cells' responses to the mitogens whereas responses to the antigens showed continued enhancement. This effect was removed by prior heat-inactivation of the complement. It persisted when the cells were exposed to C1, C4 and C2 for 1 hr, then washed and cultured with mitogen. It required active C1 in the fluid-phase prior to addition of C4 and C2. It correlated with the amount of activated C2 to which the cells were exposed. Enriched populations of T and B cells were affected equally.
最近的数据显示补体与淋巴细胞之间存在明确的关系。遗传学研究表明，控制补体成分C4和C2合成的基因与主要组织相容性复合体之间存在紧密的连锁关系。在无补体的环境中，我们研究了纯化的人补体成分单独及各种组合对人淋巴细胞体外对有丝分裂原和抗原的增殖反应的影响。发现早期补体成分C1、C4和C2共同调节淋巴细胞对这些不同刺激的反应。一般来说，每个淋巴细胞高达1000个有效分子的剂量可增强细胞对有丝分裂原和抗原的反应。更高的剂量，即每个淋巴细胞高达3000个有效分子，会逐渐抑制细胞对有丝分裂原的反应，而对抗原的反应则持续增强。这种效应可通过补体预先热灭活消除。当细胞暴露于C1、C4和C2 1小时，然后洗涤并用有丝分裂原培养时，这种效应仍然存在。在添加C4和C2之前，液相中需要有活性的C1。它与细胞暴露的活化C2量相关。T细胞和B细胞的富集群体受到的影响相同。
