Shimada N, Ueda T, Yokoshima T, Umemura K, Shomura T
Antimicrob Agents Chemother. 1982 Dec;22(6):976-80. doi: 10.1128/AAC.22.6.976.
A radioimmunoassay system has been developed for the measurement of two major metabolites of 9,3"-diacetylmidecamycin, Mb-6 and Mb-12. A radioimmunoassay for Mb-6 was performed by using anti-Mb-6 serum and a [125I]tyramined Mb-6 derivative as a radiolabeled antigen. The labeled antigen was prepared by the chloramine T method. The antiserum was obtained from a rabbit immunized with Mb-6 conjugated to bovine serum albumin. The obtained antiserum was cross-reactive with two other metabolites of 9,3"-diacetylmidecamycin, Mb-2 and Mb-12, in addition to Mb-6. This Mb-6 radioimmunoassay system could detect Mb-6 concentrations as low as 100 pg/ml of serum. The coefficients of variation were 4.5% (intra-assay) and 5.1% (inter-assay). A radioimmunoassay for Mb-12, using anti-midecamycin serum and a [125I]tyramined-Mb-12 derivative, has also been developed. The antiserum was cross-reactive only with Mb-12 among the 9,3"-diacetylmidecamycin metabolites. This Mb-12 radioimmunoassay system could detect Mb-12 concentrations as low as 2 ng/ml. The intra- and inter-assay variances were 5.9 and 5.8%, respectively.
已开发出一种放射免疫分析系统，用于测定9,3"-二乙酰麦迪霉素的两种主要代谢物Mb-6和Mb-12。通过使用抗Mb-6血清和[125I]酪胺化Mb-6衍生物作为放射性标记抗原，对Mb-6进行放射免疫分析。标记抗原采用氯胺T法制备。抗血清取自用与牛血清白蛋白偶联的Mb-6免疫的兔子。除Mb-6外，所得抗血清与9,3"-二乙酰麦迪霉素的另外两种代谢物Mb-2和Mb-12有交叉反应。这种Mb-6放射免疫分析系统能够检测低至100 pg/ml血清的Mb-6浓度。变异系数分别为4.5%（批内）和5.1%（批间）。还开发了一种使用抗麦迪霉素血清和[125I]酪胺化-Mb-12衍生物对Mb-12进行的放射免疫分析。在9,3"-二乙酰麦迪霉素代谢物中，该抗血清仅与Mb-12有交叉反应。这种Mb-12放射免疫分析系统能够检测低至2 ng/ml的Mb-12浓度。批内和批间方差分别为5.9%和5.8%。
