Auger P, Marquis G, Dallaire L, Joly J
J Lab Clin Med. 1980 Feb;95(2):272-81.
The interaction of human AF with the pathogenic yeast Candida albicans was studied in vitro in 262 samples from early midtrimester and term gestations. Among 111 fluids collected between 15 and 18 weeks, 84% (93) manifested growth-inhibiting activity on 10(2) fungal cells/ml at 24 hr. This phenomenon persisted to a similar extent in late pregnancy. It could not be explained by a lack of nutrients in AF, since the addition of TSB failed to influence the proportion of active fluids. Depletion of the yeast cell glycogen reserve potentiated the degree of fungistasis achieved in several samples (p = 0.0009). Supplementation with either SDB or a physiologic concentration of carbon dioxide effected a complete reversal (p less than 0.0001). Preliminary data as well as overall results suggest that the absolute transferrin content and its pH-dependent saturation with iron are key factors invovled in the growth-inhibiting activity. Germ tube formation was unrestricted in 115 second trimester fluids studied. Human AF probably helps to prevent congenital candidiasis, and this protective function must be better defined.
在体外研究了来自妊娠中期早期和足月妊娠的262份样本中人类羊水（AF）与致病性酵母白色念珠菌的相互作用。在15至18周收集的111份液体中，84%（93份）在24小时时对每毫升10²个真菌细胞表现出生长抑制活性。这种现象在妊娠后期持续存在且程度相似。这不能用羊水中缺乏营养来解释，因为添加胰酪大豆胨肉汤（TSB）未能影响具有活性的液体比例。酵母细胞糖原储备的耗尽增强了几个样本中实现的抑菌程度（p = 0.0009）。补充大豆消化酪蛋白肉汤（SDB）或生理浓度的二氧化碳可实现完全逆转（p < 0.0001）。初步数据以及总体结果表明，转铁蛋白的绝对含量及其与铁的pH依赖性饱和度是参与生长抑制活性的关键因素。在所研究的115份妊娠中期液体中，芽管形成不受限制。人类羊水可能有助于预防先天性念珠菌病，这种保护功能必须得到更好的界定。
