成年胰岛细胞在经渗透压破坏的成纤维细胞上的单层培养。
Monolayer cultures of adult pancreatic islet cells on osmotically disrupted fibroblasts.
作者信息
Meda P, Hooghe-Peters E L, Orci L
出版信息
Diabetes. 1980 Jun;29(6):497-500. doi: 10.2337/diab.29.6.497.
PMID:6991337
Abstract
A simple method that permits the attachment of fully dissociated adult pancreatic islet cells to culture dishes, using osmotically disrupted fibroblasts, is described. Using this system, monolayer cultures consisting of either isolated or clustered adult endocrine islet cells can be readily established in standard culture media.
摘要
本文描述了一种简单的方法，该方法利用经渗透压破坏的成纤维细胞，使完全解离的成年胰腺胰岛细胞附着于培养皿。利用该系统，可在标准培养基中轻松建立由分离的或聚集的成年内分泌胰岛细胞组成的单层培养物。
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