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成年胰岛细胞在经渗透压破坏的成纤维细胞上的单层培养。

Monolayer cultures of adult pancreatic islet cells on osmotically disrupted fibroblasts.

作者信息

Meda P, Hooghe-Peters E L, Orci L

出版信息

Diabetes. 1980 Jun;29(6):497-500. doi: 10.2337/diab.29.6.497.

DOI:10.2337/diab.29.6.497
PMID:6991337
Abstract

A simple method that permits the attachment of fully dissociated adult pancreatic islet cells to culture dishes, using osmotically disrupted fibroblasts, is described. Using this system, monolayer cultures consisting of either isolated or clustered adult endocrine islet cells can be readily established in standard culture media.

摘要

本文描述了一种简单的方法，该方法利用经渗透压破坏的成纤维细胞，使完全解离的成年胰腺胰岛细胞附着于培养皿。利用该系统，可在标准培养基中轻松建立由分离的或聚集的成年内分泌胰岛细胞组成的单层培养物。

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Monolayer cultures of adult pancreatic islet cells on osmotically disrupted fibroblasts.成年胰岛细胞在经渗透压破坏的成纤维细胞上的单层培养。
Diabetes. 1980 Jun;29(6):497-500. doi: 10.2337/diab.29.6.497.
2
Insulin release from monolayer cultures of adult rat pancreatic islets.成年大鼠胰岛单层培养物中的胰岛素释放。
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Cell interactions during the in vitro neoformation of fetal rat pancreatic islets.胎鼠胰岛体外新形成过程中的细胞相互作用。
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Superfusion of dissociated pancreatic islet cells attached to Cytodex beads.附着于Cytodex微珠上的解离胰岛细胞的灌注。
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Factors from fibroblasts promote pancreatic islet B cell survival in tissue culture.来自成纤维细胞的因子在组织培养中促进胰岛B细胞存活。
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[Ultrastructural characteristics of a monolayer cell culture of the guinea pig pancreatic islet apparatus].[豚鼠胰岛装置单层细胞培养的超微结构特征]
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Long-term culture of isolated pancreatic islet cells.
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Promotion of monolayer formation in cultured whole pancreatic islets by 3-isobutyl-1-methylxanthine.3-异丁基-1-甲基黄嘌呤促进培养的全胰腺胰岛单层形成。
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[Cytological and insulin-producing characteristics of cultures of pancreatic islet cells from newborn piglets].[新生仔猪胰岛细胞培养物的细胞学及胰岛素分泌特性]
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