Angerman C R, Eisenstein T K
Infect Immun. 1980 Feb;27(2):435-43. doi: 10.1128/iai.27.2.435-443.1980.
Groups of mice were immunized with optimal doses of the following vaccines of Salmonella typhimurium W118-2: acetone-killed cells, lipopolysaccharide, ribosomes, and live cells. At 3 weeks, 1 month, 2 months, 4 months, or 6 months postimmunization, sera were collected from control and vaccinated animals, and the anti-lipopolysaccharide and whole-cell agglutination titers of the sera were determined. Other groups of similarly vaccinated mice were tested for resistance to infection by challenging with live W118-2 and scoring the number of survivors 30 days postinfection. It was found that only ribosomes and live cells afforded significant protection 6 months after immunization. Thus, in duration of protection ribosomes were superior to the other nonviable vaccines tested. At all time intervals tested, purified lipopolysaccharide was the least effective vaccine. Protection afforded by the acetone-killed cell and ribosomal vaccines correlated better with the whole-cell agglutination titers than with the anti-lipopolysaccharide titers. However, the longer duration of protection afforded by the ribosomal vaccine, as compared with the acetone-killed vaccine, could not be accounted for by differences in whole-cell agglutination titers. These studies show that ribosomal vaccines are equal in all parameters to acetone-killed cells and have the advantage of providing longer-lasting immunity.
将小鼠分成几组，用鼠伤寒沙门氏菌W118 - 2的以下疫苗的最佳剂量进行免疫：丙酮灭活菌、脂多糖、核糖体和活菌体。在免疫后3周、1个月、2个月、4个月或6个月，从对照动物和接种疫苗的动物中采集血清，并测定血清的抗脂多糖和全细胞凝集效价。用其他同样接种疫苗的小鼠组通过用活的W118 - 2攻击并记录感染后30天存活者的数量来测试其对感染的抵抗力。结果发现，只有核糖体和活菌体在免疫6个月后提供了显著的保护。因此，在保护持续时间方面，核糖体优于所测试的其他非活疫苗。在所有测试的时间间隔内，纯化的脂多糖是最无效的疫苗。丙酮灭活菌疫苗和核糖体疫苗提供的保护与全细胞凝集效价的相关性比与抗脂多糖效价的相关性更好。然而，与丙酮灭活疫苗相比，核糖体疫苗提供的更长保护持续时间不能用全细胞凝集效价的差异来解释。这些研究表明，核糖体疫苗在所有参数上与丙酮灭活菌相当，并且具有提供更持久免疫力的优势。
