Metcalf E S, O'Brien A D
Infect Immun. 1981 Jan;31(1):33-41. doi: 10.1128/iai.31.1.33-41.1981.
A heavy-chain class-specific, solid-phase radioimmunoassay was developed to characterize the murine antibody response to Salmonella typhimurium. The specificity of the assay was verified by quantitation of the extent of binding of anti-S. typhimurium antibodies to other bacterial genera and species and by cross-adsorption studies. The sensitivity of the procedure was also examined, and it was determined to be substantially more sensitive than either the passive hemagglutination or the whole-cell agglutination technique. The method was subsequently used to analyze th murine antibody response to S. typhimurium. Groups of mice were prebled and then immunized with live S. typhimurium via different routes. The animals were bled weekly for 12 weeks, and then sera were assayed for antibodies directed against whole bacteria or purified lipopolysaccharide. Anti-Salmonella antibodies of the immunoglobulin M class appeared in the serum approximately 2 to 3 weeks after immunization, and then immunoglobulin G anti-Salmonella antibodies appeared which constituted the major part of the long-term response. Immunoglobulin A was not a major component of the serum antibody response. The antibodies were primarily directed against the lipopolysaccharide determinants, but a small percentage of the response was directed against other cell surface components. Qualitatively and quantitatively similar anti-Salmonella antibody responses were observed in sera of outbred and inbred strains of mice.
开发了一种重链类别特异性固相放射免疫测定法，以表征小鼠对鼠伤寒沙门氏菌的抗体反应。通过定量抗鼠伤寒沙门氏菌抗体与其他细菌属和种的结合程度以及交叉吸附研究，验证了该测定法的特异性。还检查了该方法的灵敏度，确定其比被动血凝或全细胞凝集技术灵敏得多。随后使用该方法分析小鼠对鼠伤寒沙门氏菌的抗体反应。将小鼠分组预先采血，然后通过不同途径用活的鼠伤寒沙门氏菌免疫。每周对动物采血，持续12周，然后检测血清中针对全菌或纯化脂多糖的抗体。免疫球蛋白M类抗沙门氏菌抗体在免疫后约2至3周出现在血清中，然后出现免疫球蛋白G抗沙门氏菌抗体，其构成长期反应的主要部分。免疫球蛋白A不是血清抗体反应的主要成分。抗体主要针对脂多糖决定簇，但一小部分反应针对其他细胞表面成分。在远交系和近交系小鼠的血清中观察到定性和定量相似的抗沙门氏菌抗体反应。
