Keller M A, Turner J L, Stratton J A, Miller M E
Infect Immun. 1980 Mar;27(3):903-9. doi: 10.1128/iai.27.3.903-909.1980.
Comparison milk and blood lymphocyte blastogenic responses to the K1 antigen of Escherichia coli and lipopolysaccharide (LPS) from E. coli O127,B8 were examined in 16 postpartum women by [3H]thymidine uptake. Rabbit hemolysincoated sheep erythrocyte monolayers were used to deplete macrophages from milk lymphocyte preparations and to enrich for T lymphocytes in order to make milk preparations more comparable to blood preparations. Response was defined as a stimulation index of greater than or equal to 2.0. There was no evidence of selective response to K1 antigen by milk lymphocytes, since both blood and milk lymphocytes responded in four women and neither blood nor milk lymphocytes responded in nine. Milk lymphocytes alone responded to K1 in one woman, whereas blood lymphocytes alone responded in two women. Additional nonpaired milk or blood cultures were available from three women. None of these responded to K1 antigen. Corresponding lymphocyte cultures were stimulated with LPS. A positive K1 response was always accompanied by an LPS response, and the LPS response correlated with the K1 response in 17 of 19 women. Stool cultures examined with an antiserum agar showed no correlation between the presence of K1 E. coli in the stool and milk or blood lymphocyte response to K1 antigen. In the system used here, no selectivity of response of breast milk lymphocytes to K1 antigen was noted.
通过[3H]胸腺嘧啶核苷摄取法，检测了16名产后妇女的乳汁和血液淋巴细胞对大肠杆菌K1抗原及大肠杆菌O127:B8脂多糖（LPS）的促有丝分裂反应。用兔溶血素包被的绵羊红细胞单层来清除乳汁淋巴细胞制剂中的巨噬细胞并富集T淋巴细胞，以使乳汁制剂更可比血液制剂。反应定义为刺激指数大于或等于2.0。没有证据表明乳汁淋巴细胞对K1抗原有选择性反应，因为4名妇女的血液和乳汁淋巴细胞均有反应，9名妇女的血液和乳汁淋巴细胞均无反应。仅1名妇女的乳汁淋巴细胞对K1有反应，而仅2名妇女的血液淋巴细胞对K1有反应。3名妇女有额外的非配对乳汁或血液培养物。这些均未对K1抗原产生反应。相应的淋巴细胞培养物用LPS刺激。K1阳性反应总是伴有LPS反应，并且在19名妇女中的17名中，LPS反应与K1反应相关。用抗血清琼脂检测粪便培养物显示，粪便中K1大肠杆菌的存在与乳汁或血液淋巴细胞对K1抗原的反应之间没有相关性。在此处使用的系统中，未观察到母乳淋巴细胞对K1抗原反应的选择性。
