Oellerich M, Engelhardt P, Schaadt M, Diehl V
J Clin Chem Clin Biochem. 1980 Mar;18(3):169-74. doi: 10.1515/cclm.1980.18.3.169.
A homogeneous enzyme immunoassay for the determination of methotrexate in serum (EMIT, Syva Corp.) was fully mechanized by the use of an Eppendorf analyzer 5010. Coefficients of variation from day to day were in the range 2--16%. A comparison of the results obtained by EMIT and a radioimmunoassay (Methotrexate 125 I Radioimmunoassay Kit, Diagnostic Biochemistry Inc.) in a series of 50 samples from patients showed a good correlation between both methods. The specificity of the EMIT assay appeared to be adequate. The time required for the determination of about 20 patient samples by this procedure was only about 80 minutes. From our clinical experimence the EMIT assay appears to be well suited for routine monitoring of methotrexate serum concentrations above 0.1 mumol/1 during intrathecal and high dose methotrexate therapy.
采用Eppendorf 5010分析仪，实现了用于测定血清中甲氨蝶呤的均相酶免疫分析方法（EMIT，Syva公司）的全自动化。其日间变异系数在2%至16%之间。对50例患者的一系列样本采用EMIT法和放射免疫分析法（甲氨蝶呤125I放射免疫分析试剂盒，Diagnostic Biochemistry公司）所得结果进行比较，结果表明两种方法具有良好的相关性。EMIT分析的特异性似乎足够。用此方法测定约20份患者样本所需时间仅约80分钟。根据我们的临床经验，EMIT分析似乎非常适合在鞘内注射和高剂量甲氨蝶呤治疗期间对血清中甲氨蝶呤浓度高于0.1μmol/L的情况进行常规监测。
