Lederman H M, Lee J W, Cheung R K, Grinstein S, Gelfand E W
Proc Natl Acad Sci U S A. 1984 Nov;81(21):6827-30. doi: 10.1073/pnas.81.21.6827.
We have used the T-cell mitogen Staphylococcus aureus protein A (SpA) to study the role of monocytes in the early events of T-lymphocyte activation. The mitogenic response of human peripheral blood mononuclear cells (PBM) was compared to the response of populations enriched for T cells by E-rosetting (PBM-E+). In response to SpA, the [3H]thymidine uptake of PBM-E+ was reduced by 80% compared to PBM. The reduced response of PBM-E+ was completely restored by the addition of irradiated PBM-E- or the monocyte-like human cell line U-937 but not by addition of irradiated PBM-E+. A direct interaction of SpA with monocytes is important since proliferative responses could be generated by preincubation of U-937 with SpA followed by washing and subsequent addition to PBM-E+; incubation of PBM-E+ with SpA followed by washing and subsequent addition of U-937 did not result in a proliferative response. To further delineate the role of the monocyte, we examined the ability of soluble SpA, U-937, or U-937 preincubated with SpA to trigger Ca2+ flux into T lymphocytes, an early step in initiation of the proliferative response. SpA-pretreated U-937, but neither SpA nor monocytes alone, triggered Ca2+ movement into the T lymphocytes. This defines a new role for the monocyte in the early events of T-lymphocyte activation.
我们利用T细胞促有丝分裂原金黄色葡萄球菌蛋白A（SpA）来研究单核细胞在T淋巴细胞激活早期事件中的作用。将人外周血单个核细胞（PBM）的促有丝分裂反应与通过E花环富集T细胞的群体（PBM-E+）的反应进行比较。响应SpA时，PBM-E+的[3H]胸苷摄取量与PBM相比降低了80%。添加经辐照的PBM-E-或单核细胞样人细胞系U-937可完全恢复PBM-E+降低的反应，但添加经辐照的PBM-E+则不能。SpA与单核细胞的直接相互作用很重要，因为U-937与SpA预孵育，然后洗涤，随后添加到PBM-E+中可产生增殖反应；PBM-E+与SpA孵育，然后洗涤，随后添加U-937则不会导致增殖反应。为了进一步阐明单核细胞的作用，我们检测了可溶性SpA、U-937或与SpA预孵育的U-937触发Ca2+流入T淋巴细胞的能力，这是增殖反应启动的早期步骤。经SpA预处理的U-937，但单独的SpA或单核细胞均不能触发Ca2+流入T淋巴细胞。这确定了单核细胞在T淋巴细胞激活早期事件中的新作用。
