Pontefract R D, Bergeron G
Appl Environ Microbiol. 1985 Feb;49(2):456-8. doi: 10.1128/aem.49.2.456-458.1985.
A method is described that improved the detection of viruses in fecal samples by electron microscopy. The virus particles were concentrated, and much of the background debris was removed by adsorption of viruses on meat protein added to the fecal sample at a low pH and a low salt concentration. Viruses were eluted by raising the pH and the salt concentration. Further concentration was achieved by acid precipitation and vacuum dialysis.
本文描述了一种通过电子显微镜改进粪便样本中病毒检测的方法。病毒颗粒被浓缩，通过在低pH值和低盐浓度下将病毒吸附到添加到粪便样本中的肉类蛋白上，去除了大部分背景碎片。通过提高pH值和盐浓度洗脱病毒。通过酸沉淀和真空透析进一步浓缩。
