Williams-Boyce P K, Daniel J C
J Exp Zool. 1980 May;212(2):243-53. doi: 10.1002/jez.1402120211.
Holes punched in the ears of rabbits are repaired by regeneration of new tissues from blastemas found on the periphery of the wounds. The proportion whch successfully regenerated was greater in males than in females (75% vs 20%), in pregnancy than during etrus or lactation (75% vs 25%), and in ovariectomized does given testosterone than in those given oil carrier alone (88% vs 50%), but the speed of closure did not differ in these groups. The steroid hormone influence postulated by other workers is confirmed. Closure was faster in younger animals and for second holes punched on the same site where earlier holes had been repaired, in both cases, by shortening of the initial seven to ten day delay period seen for primary holes in adult ears. The cartilage layer was also thicker after regeneration from secondary punches and the success rate greater (67% vs 29%). These observations suggest a "priming" effect on the tissue from the primary punch. Ear holes in the area proximal to the head were repaired faster and with more success (78% vs 12%) than those punched distally. The relative thickness of the cartilage layer may be the critical factor. Tissues did not regenerate from semicircular layer may be the critical factor. Tissues did not regenerate from semicircular wounds made on the edge of the pinna. Hair grown on regenerated ear skin reestablished the original color pattern. Skin regenerating for hles punched where skin from the back had been transplanted to ears had the characteristics of back skin, but no regeneration was detectable from transplanted toe tissue. Skin from other locations can regenerate in the environment provided by the ear but more complex tissue structures put into the same locatin apparently cannot. Skin cells involved in regeneration originate from the tissues bounding the wound.
在兔子耳朵上打的洞，是通过伤口周边发现的芽基再生新组织来修复的。成功再生的比例在雄性中比雌性更高（75%对20%），在怀孕期比发情期或哺乳期更高（75%对25%），在接受睾酮的去卵巢母兔中比仅接受油载体的母兔更高（88%对50%），但这些组的闭合速度没有差异。其他研究者推测的类固醇激素影响得到了证实。在较年轻的动物中闭合更快，并且对于在同一部位打的第二个洞（此处先前的洞已修复），在这两种情况下，通过缩短成年耳朵上初次打孔时最初出现的7至10天延迟期，闭合也更快。从二次打孔再生后软骨层也更厚，成功率更高（67%对29%）。这些观察结果表明初次打孔对组织有“启动”作用。靠近头部区域的耳洞比在远端打的耳洞修复得更快且成功率更高（78%对12%）。软骨层的相对厚度可能是关键因素。从耳廓边缘的半圆形伤口处组织不会再生。再生耳皮肤上长出的毛发重新形成了原来的颜色图案。在将背部皮肤移植到耳朵上打孔处再生的皮肤具有背部皮肤的特征，但从移植的脚趾组织处未检测到再生。来自其他部位的皮肤可以在耳朵提供的环境中再生，但显然放入同一位置的更复杂组织结构不能再生。参与再生的皮肤细胞起源于伤口周围的组织。
