Pagé M, Thériault L, Nilsson M
Scand J Clin Lab Invest. 1980;40(7):641-5. doi: 10.1080/00365518009091976.
We describe a solid-phase enzyme immunoassay for human serum ferritin, with the use of polyethylene glycol to shorten the reaction. The amount of bound enzyme-antibody conjugate used as a second site antibody is proportional to the ferritin titre in the assay. This method is fast, and the reagents are stable for many months at 4 degrees C. The lowest detectable concentration was 5 micrograms/l. Interassay coefficients of variation were 9.3 and 12.2%, respectively, at ferritin concentrations of 100 micrograms/l and 200 micrograms/l. The data also show that polyethylene glycol accelerates antigen-antibody complex formation in the solid phase. An assay may be completed in 5 h.
我们描述了一种用于人血清铁蛋白的固相酶免疫测定法，使用聚乙二醇来缩短反应时间。用作第二位点抗体的结合酶 - 抗体缀合物的量与测定中的铁蛋白滴度成正比。该方法快速，试剂在4℃下可稳定保存数月。最低可检测浓度为5微克/升。在铁蛋白浓度为100微克/升和200微克/升时，批间变异系数分别为9.3%和12.2%。数据还表明聚乙二醇可加速固相中抗原 - 抗体复合物的形成。该测定可在5小时内完成。
