Bell W M, Chaffin W L
Can J Microbiol. 1980 Jan;26(1):102-5. doi: 10.1139/m80-015.
The yeast-mycelial transition in Candida albicans can be induced from yeast cells grown on minimal defined medium only in stationary phase. This study examined the inducibility of cultures in which growth was limited by the availability of the nutrients, glucose, NH4Cl, or galactose. The results showed that neither stationary phase nor cell cycle stage alone was a sufficient condition to support subsequent germ tube formation. In addition, final cell concentration alone was not a factor in inducibility. When a hundredfold decrease in growth was obtained by limiting any of the nutrients, a loss in inducibility was observed. However, the loss of inducibility differed with the limiting nutrient. Galactose, NH4Cl, and glucose-limited cultures showed respectively 15, 30, and 80% loss of inducibility. Thus the effect was associated with both carbon/energy and nitrogen-limited cells; however, glucose appeared to have a specific effect. These observations suggest that the metabolic state of the stationary phase yeast cell was an important factor in the subsequent ability to respond to conditions inducing germ tube formation.
白色念珠菌的酵母-菌丝体转变只能在仅在稳定期生长于限定基本培养基上的酵母细胞中诱导产生。本研究检测了生长受营养物质（葡萄糖、氯化铵或半乳糖）可用性限制的培养物的诱导能力。结果表明，单独的稳定期或细胞周期阶段都不是支持后续芽管形成的充分条件。此外，单独的最终细胞浓度也不是诱导能力的一个因素。当通过限制任何一种营养物质使生长降低一百倍时，观察到诱导能力丧失。然而，诱导能力的丧失因限制的营养物质而异。半乳糖、氯化铵和葡萄糖限制的培养物分别显示出15%、30%和80%的诱导能力丧失。因此，这种影响与碳/能量和氮限制的细胞都有关；然而，葡萄糖似乎有特定的影响。这些观察结果表明，稳定期酵母细胞的代谢状态是其后续对诱导芽管形成条件作出反应能力的一个重要因素。
