Freedman V H, Calvelli T A, Silagi S, Silverstein S C
J Exp Med. 1980 Sep 1;152(3):657-73. doi: 10.1084/jem.152.3.657.
We have demonstrated that a murine cytotoxic peritoneal cell can be elicited by intraperitoneal immunization with heat-killed Mycobacterium bovis, strain Bacillus Calmette-Guérin (BCG). When these cells are injected together with cells of clone B(5)59 of B16 melanoma in a Winn-type transfer assay into syngeneic C57BL/6J mice, the tumorigenic potential of the melanoma is completely abrogated. Similarly, mice immunized intraperitoneally with dead BCG are protected against intraperitoneal challenge with a number of B16 melanoma cells sufficient to cause tumors in 100% of control mice. However, mice immunized intraperitoneally with dead BCG are not protected against tumor formation when B16 melanoma cells are injected subcutaneously. Co-injection of BCG-elicited peritoneal cells with B16 melanoma cells into nude or sublethally irradiated (650 rad) mice inhibits tumor formation in > 85% of the mice, indicating that additional participation of host bone marrow- or thymus-derived leukocytes is not required to eradicate the tumor implant. The effector cell in the BCG-induced peritoneal exudate is adherent and phagocytic and is a mononuclear phagocyte. Nonadherent lymphoid cells from the same BCG-induced peritoneal exudate and from thioglycollate-broth-elicited granylocytes and macrophages neither prevent nor delay B16 tumor formation.
我们已经证明，通过用热灭活的牛分枝杆菌卡介苗（BCG）进行腹腔内免疫，可以诱导出小鼠细胞毒性腹膜细胞。当在Winn型转移试验中将这些细胞与B16黑色素瘤的B(5)59克隆细胞一起注射到同基因C57BL/6J小鼠中时，黑色素瘤的致瘤潜力被完全消除。同样，用死卡介苗进行腹腔内免疫的小鼠可免受一定数量的B16黑色素瘤细胞的腹腔内攻击，这些细胞足以使100%的对照小鼠发生肿瘤。然而，当皮下注射B16黑色素瘤细胞时，用死卡介苗进行腹腔内免疫的小鼠不能免受肿瘤形成的影响。将卡介苗诱导的腹膜细胞与B16黑色素瘤细胞共同注射到裸鼠或亚致死剂量照射（650拉德）的小鼠中，可抑制>85%的小鼠形成肿瘤，这表明根除肿瘤植入物不需要宿主骨髓或胸腺来源的白细胞的额外参与。卡介苗诱导的腹膜渗出物中的效应细胞具有黏附性和吞噬性，是一种单核吞噬细胞。来自相同卡介苗诱导的腹膜渗出物以及来自硫乙醇酸盐肉汤诱导的粒细胞和巨噬细胞的非黏附性淋巴细胞既不能预防也不能延迟B16肿瘤的形成。
