LeBouton A V, Masse J P
Anat Rec. 1980 Jun;197(2):195-201. doi: 10.1002/ar.1091970208.
The cellular immunolocalization of albumin in rat liver has been studied as a function of various physiological and physical conditions. Our observations show that the prime requisite for accurate immunolocalization of albumin and other hepatic-based proteins is the complete removal of blood and especially plasma from sinusoids and the perisinusoidal space of Disse prior to fixation. Fixation of blood-filled liver specimens results in the antifactual entrance of plasma constituents into hepatocytes. When the fixative used in formaldehyde, the artifactual uptake occurs primarily into hepatocytes that have a high glycogen content. Fixation of blood-filled liver with acetic acid-ethanol causes a massive influx of plasma into all hepatocytes. On the contrary, with blood-free liver, varying the type of fixative consistently demonstrates that all hepatocytes normally contain albumin, transferrin, and fibrinogen simultaneously. Increasing the time between cessation of blood flow and outright fixation by either withholding the fixative or by impeding its diffusion through the specimen causes a progressive loss of antigenicity of albumin. The same result ensues when specimens remain in contact with the fixative for an extended time.
已研究了大鼠肝脏中白蛋白的细胞免疫定位与各种生理和物理条件的关系。我们的观察结果表明，准确免疫定位白蛋白和其他肝脏蛋白的首要条件是在固定前完全清除血窦和狄氏间隙中的血液，尤其是血浆。固定充满血液的肝脏标本会导致血浆成分人为地进入肝细胞。当使用甲醛作为固定剂时，人为摄取主要发生在糖原含量高的肝细胞中。用醋酸 - 乙醇固定充满血液的肝脏会导致血浆大量涌入所有肝细胞。相反，对于无血肝脏，改变固定剂类型始终表明所有肝细胞通常同时含有白蛋白、转铁蛋白和纤维蛋白原。通过延迟固定剂或阻碍其在标本中的扩散来增加血流停止与直接固定之间的时间，会导致白蛋白抗原性逐渐丧失。当标本与固定剂长时间接触时也会出现相同的结果。
