Fitzgerald T J, Johnson R C, Wolff E T
Br J Vener Dis. 1980 Jun;56(3):129-36. doi: 10.1136/sti.56.3.129.
Certain reducing agents containing sulfhydryl groups are important to the in-vitro survival of Treponema pallidum. Discrepancies occur, however, concerning the agents and the concentrations that are optimal. To clarify some of this confusion, sulfhydryl oxidation was determined using procedures and experimental conditions commonly used for T pallidum. Sulfhydryl oxidation varied according to the type of culture medium, the size of the culture vessels, the volume of the culture medium, and the gaseous environment within the culture vessels, as well as the method of extracting treponmes from infected testicular tissue. Dithiothreotol maintained highly reduced conditions by reducing disulfide groups to sulfhydryl groups. Lastly, the organisms influenced the sulfhydryl concentration by either direct oxidation or specific uptake. The sulfhydryl content was sharply decreased in the presence of viable preparations of T pallidum compared with heated preparations or membrane filtrates of viable preparations.
某些含巯基的还原剂对梅毒螺旋体的体外存活很重要。然而，关于最佳的试剂和浓度存在差异。为了澄清其中的一些混淆，使用常用于梅毒螺旋体的程序和实验条件来测定巯基氧化。巯基氧化因培养基类型、培养容器大小、培养基体积、培养容器内的气体环境以及从感染睾丸组织中提取梅毒螺旋体的方法而异。二硫苏糖醇通过将二硫键还原为巯基来维持高度还原的条件。最后，这些生物体通过直接氧化或特异性摄取来影响巯基浓度。与加热制剂或活体制剂的膜滤液相比，在活的梅毒螺旋体制剂存在下，巯基含量急剧下降。
