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大质粒在豆科根瘤菌内共生形式中的表达。

Expression of large plasmids in the endosymbiotic form of Rhizobium leguminosarum.

作者信息

Krol A J, Hontelez J G, Van den Bos R C, Van Kammen A

出版信息

Nucleic Acids Res. 1980 Oct 10;8(19):4337-47. doi: 10.1093/nar/8.19.4337.

DOI:10.1093/nar/8.19.4337
PMID:7001365
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC324243/
Abstract

Isolated plasmid DNA from Rhizobium leguminosarum was hybridised with cellular RNA from broth-cultured bacteria and endosymbiotic bacteroids. From these hybridisation, experiments it is concluded that plasmid genes are strongly expressed in bacteroids and only weakly or not at all in bacteria. From the hybridisation of plasmid DNA with the cloned structural nif genes of Klebsiella pneumoniae it is shown that at least part of the nif genes are located on a plasmid.

摘要

从豆科根瘤菌中分离出的质粒DNA与肉汤培养细菌和共生类菌体的细胞RNA进行杂交。从这些杂交实验得出结论，质粒基因在类菌体中强烈表达，而在细菌中仅微弱表达或根本不表达。通过质粒DNA与肺炎克雷伯菌克隆的结构固氮基因的杂交表明，至少部分固氮基因位于一个质粒上。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352b/324243/c3ca93bda15d/nar00436-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352b/324243/1b92afb0e162/nar00436-0027-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352b/324243/beb05da838f2/nar00436-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352b/324243/e94b020efeec/nar00436-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352b/324243/c3ca93bda15d/nar00436-0031-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352b/324243/1b92afb0e162/nar00436-0027-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352b/324243/beb05da838f2/nar00436-0029-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352b/324243/e94b020efeec/nar00436-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/352b/324243/c3ca93bda15d/nar00436-0031-a.jpg

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本文引用的文献

1
Interspecies homology of nitrogenase genes.固氮酶基因的种间同源性。
Proc Natl Acad Sci U S A. 1980 Jan;77(1):191-5. doi: 10.1073/pnas.77.1.191.
2
A membrane-filter technique for the detection of complementary DNA.一种用于检测互补DNA的膜过滤技术。
Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6. doi: 10.1016/0006-291x(66)90447-5.
3
General method for the isolation of plasmid deoxyribonucleic acid.质粒脱氧核糖核酸的分离通用方法。
Mol Gen Genet. 1981;184(2):326-33. doi: 10.1007/BF00272926.
4
On the operon structure of the nitrogenase genes of Rhizobium leguminosarum and Azotobacter vinelandii.关于豌豆根瘤菌和棕色固氮菌固氮酶基因的操纵子结构
Nucleic Acids Res. 1982 Jul 24;10(14):4147-57. doi: 10.1093/nar/10.14.4147.
5
Clustering of nitrogen fixation (nif) genes in Rhizobium meliloti.苜蓿根瘤菌中固氮（nif）基因的聚类
J Bacteriol. 1982 Jan;149(1):221-8. doi: 10.1128/jb.149.1.221-228.1982.
6
Characterization of a Rhizobium meliloti fixation gene (fixF) located near the common nodulation region.对位于常见结瘤区域附近的苜蓿根瘤菌固氮基因（fixF）的特性分析。
J Bacteriol. 1985 Oct;164(1):245-54. doi: 10.1128/jb.164.1.245-254.1985.
7
Evidence for plasmid- and chromosome-borne multiple nif genes in Rhizobium fredii.费氏根瘤菌中质粒和染色体携带的多个固氮基因的证据。
Appl Environ Microbiol. 1985 Jul;50(1):41-4. doi: 10.1128/aem.50.1.41-44.1985.
8
Characterization and nucleotide sequence of a novel gene fixW upstream of the fixABC operon in Rhizobium leguminosarum.豆科根瘤菌中fixABC操纵子上游一个新基因fixW的特性及核苷酸序列
Mol Gen Genet. 1989 Sep;218(3):536-44. doi: 10.1007/BF00332421.
J Bacteriol. 1973 Nov;116(2):1064-6. doi: 10.1128/jb.116.2.1064-1066.1973.
4
Detection of specific sequences among DNA fragments separated by gel electrophoresis.在通过凝胶电泳分离的DNA片段中检测特定序列。
J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
5
A freeze-squeeze method for recovering long DNA from agarose gels.一种从琼脂糖凝胶中回收长链DNA的冻挤法。
Anal Biochem. 1975 May 26;66(1):213-20. doi: 10.1016/0003-2697(75)90739-3.
6
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J Mol Biol. 1977 Jun 15;113(1):237-51. doi: 10.1016/0022-2836(77)90052-3.
7
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[Demonstration of extrachromosomal DNA in Rhizobium meliloti].[苜蓿根瘤菌中染色体外DNA的证明]
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9
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Nature. 1978 Aug 17;274(5672):715-7. doi: 10.1038/274715a0.
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Biochim Biophys Acta. 1978 Feb 13;539(1):1-11. doi: 10.1016/0304-4165(78)90115-0.